Takács J, Borostyánkõi Z A, Veisenberger E, Vastagh C, Víg J, Görcs T J, Hámori J
Neurobiology Research Group, United Research Organisation of the Hungarian Academy of Sciences and Semmelweis University, Budapest, Hungary.
J Neurosci Res. 2000 Jul 1;61(1):107-15. doi: 10.1002/1097-4547(20000701)61:1<107::AID-JNR13>3.0.CO;2-J.
The postnatal developmental distribution pattern of metabotropic glutamate receptor (mGluR1a) immunoreactive unipolar brush cells (UBCs) was studied in the cerebellar cortex of kittens. On the day of birth (P0) UBCs are already present in the white matter in lobule X of the vermis, but only a few of these cell seemed to migrate to the deeper region of the internal granular layer. By the end of the first week (P8) UBCs were seen to invade the white matter + internal granular layer of lobules IX, VIII, I, and II of the vermis, and they spread further in the transitory area medio-laterally from the vermis toward the cerebellar hemispheres. By P15, UBCs appeared in lobules III and VII of the vermis, as well as in corresponding lobules of the neocerebellum, with especially high numbers in lobule VII. By P22, UBCs migrated further after their medio-lateral course in the neocerebellum, and began to invade lobules V and VI. At P62 the amount of UBCs in midsagittal planes of early developing vermal lobules (I, II, VII-X) resembled the P132 or adult pattern. The medio-lateral migration and incorporation of UBCs into the late-developing cerebellar lobules V and VI was completed only by P132, when the spatial distribution of UBCs in both the vermal and neocerebellar lobules was comparable to that seen in the 1 year old young adult cat. Although by P132 the postnatal migration of the vast majority of UBCs seemed to be completed, in the cerebellum of adult cats a few migrating UBCs could still be observed in the white matter of the cerebellar lobules, and beneath the ependyma of the fourth ventricle. It is concluded that during ontogenesis the migration course of UBCs follows essentially the developmental sequence of cerebellar lobules, although the incorporation of UBCs into the internal granular layer continues until 4 months postnatally, i.e., much beyond the apparent completion (about two months postnatally) of cytoarchitectonic built up of the cerebellar cortex of kittens.
研究了新生小猫小脑皮质中代谢型谷氨酸受体（mGluR1a）免疫反应性单极刷状细胞（UBCs）的产后发育分布模式。出生当天（P0），UBCs已存在于蚓部小叶X的白质中，但这些细胞中只有少数似乎迁移到内颗粒层的较深区域。到第一周结束时（P8），可见UBCs侵入蚓部小叶IX、VIII、I和II的白质+内颗粒层，并从蚓部向小脑半球在中侧的过渡区域进一步扩散。到P15时，UBCs出现在蚓部小叶III和VII以及新小脑的相应小叶中，在小叶VII中数量尤其多。到P22时，UBCs在新小脑中沿中侧方向进一步迁移，并开始侵入小叶V和VI。在P62时，早期发育的蚓部小叶（I、II、VII - X）矢状面中的UBCs数量类似于P132或成年模式。UBCs向中侧迁移并融入发育较晚的小脑小叶V和VI的过程直到P132才完成，此时UBCs在蚓部和新小脑小叶中的空间分布与1岁年轻成年猫中的情况相当。尽管到P132时绝大多数UBCs的产后迁移似乎已经完成，但在成年猫的小脑中，仍可在小脑小叶的白质以及第四脑室室管膜下方观察到少数正在迁移的UBCs。得出的结论是，在个体发育过程中，UBCs的迁移过程基本上遵循小脑小叶的发育顺序，尽管UBCs融入内颗粒层的过程一直持续到出生后4个月，即远远超过小猫小脑皮质细胞构筑明显完成（约出生后两个月）的时间。
