Misakian M, Kasianowicz J J
Biotechnology Division, National Institute of Standards and Technology, ACSL 227/A251, Gaithersburg, MD 20899-8313, USA.
J Membr Biol. 2003 Oct 1;195(3):137-46. doi: 10.1007/s00232-003-0615-1.
The current-voltage relationship of a single Staphylococcus aureus alpha-hemolysin (alphaHL) channel is nonlinear, rectifying, and depends on the bulk pH and the ionic strength. The data are described qualitatively by a simple one-dimensional Nernst-Planck analysis in which the fixed charges inside and near the pore's entrances affect the transport of ions through the channel. The distances of these fixed charges from one of the channel's entrances are obtained from the channel's crystal structure. The model demonstrates that rectification of monovalent ion flow through the alphaHL channel can be related to the asymmetry in the location of the ionizable amino acid side chains.
单个金黄色葡萄球菌α-溶血素（αHL）通道的电流-电压关系是非线性的、整流性的，并且取决于总体pH值和离子强度。通过简单的一维能斯特-普朗克分析对数据进行定性描述，其中孔入口内部及附近的固定电荷会影响离子通过通道的传输。这些固定电荷与通道其中一个入口的距离可从通道的晶体结构中获得。该模型表明，单价离子通过αHL通道的整流作用可能与可电离氨基酸侧链位置的不对称性有关。
