Utoh J, Zajkowski-Brown J E, Harasaki H
Cleveland Clinic Foundation, OH 44195-5132.
J Invest Surg. 1992 Oct-Dec;5(4):305-13. doi: 10.3109/08941939209012448.
It is important to know the species differences when data from animal experiments are interpreted for human application. This in vitro study focused on the effects of heat, a major concern in mechanically actuated artificial heart development, on the physiology of human and calf erythrocytes (RBC). RBC from calves and healthy human donors were incubated at 25, 37, 46, 48, 50, or 52 degrees C for 1 h. Osmotic fragility was tested and morphological changes were then observed by scanning electron microscopy. The osmotic fragility of human and calf RBC increased at and above 50 degrees C. After incubation at 50 degrees C, 6% of human and 1% of calf RBC hemolyzed. Changes in surface morphology, which included spherocytic or echinocytic forms, were observed in 97% of human and 19% of calf RBC after incubation at 50 degrees C. In conclusion, human RBC showed greater changes in osmotic fragility and morphology at and above 50 degrees C. These changes, however, were not observed in either species after 1 h incubation at 46 degrees C.
在将动物实验数据应用于人类时，了解物种差异非常重要。这项体外研究聚焦于热量（机械驱动人工心脏研发中的一个主要问题）对人和小牛红细胞（RBC）生理机能的影响。将来自小牛和健康人类供体的红细胞在25、37、46、48、50或52摄氏度下孵育1小时。测试渗透脆性，然后通过扫描电子显微镜观察形态变化。人和小牛红细胞的渗透脆性在50摄氏度及以上时增加。在50摄氏度孵育后，6%的人类红细胞和1%的小牛红细胞发生溶血。在50摄氏度孵育后，97%的人类红细胞和19%的小牛红细胞出现了包括球形或棘形等表面形态变化。总之，人类红细胞在50摄氏度及以上时，渗透脆性和形态变化更大。然而，在46摄氏度孵育1小时后，两个物种均未观察到这些变化。
