Li Wei, Thompson Carol H, Cossart Yvonne E, O'Brien Christopher J, McNeil Edward B, Scolyer Richard A, Rose Barbara R
Sydney Head and Neck Cancer Institute, PO Box M 142, Missenden Road, Camperdown, NSW 2050, Australia.
Head Neck. 2004 Jan;26(1):1-9. doi: 10.1002/hed.10335.
Chemical carcinogens induce squamous cell carcinoma (SCC) of the head and neck by targeting the p53 and the retinoblastoma (pRb) pathways. Human papillomavirus (HPV) might have an etiologic role in these cancers at particular sites. Few studies have compared cell cycle protein expression in HPV-positive and HPV-negative tumors in this region.
Fifty tonsil SCCs were analyzed for HPV by PCR and for expression of cell cycle proteins (p53, pRb, p16(INK4A), p21(CIP1/WAF1), p27(KIP1), and cyclinD1) by immunohistochemistry.
HPV was present in 42%; almost all were type 16. There were statistical associations between HPV positivity and reduced expression of pRb and cyclinD1, overexpression of p16, and younger patient age. Tumor with down-regulated p27 tended to have down-regulated pRb and p21.
HPV-positive tonsil SCCs have distinct molecular pathways. Their association with younger patient age suggests that they are biologically distinct from HPV-negative tumors.
化学致癌物通过靶向p53和视网膜母细胞瘤（pRb）通路诱导头颈部鳞状细胞癌（SCC）。人乳头瘤病毒（HPV）可能在这些特定部位的癌症中具有病因学作用。很少有研究比较该区域HPV阳性和HPV阴性肿瘤中细胞周期蛋白的表达。
通过PCR分析50例扁桃体SCC的HPV情况，并通过免疫组织化学分析细胞周期蛋白（p53、pRb、p16（INK4A）、p21（CIP1/WAF1）、p27（KIP1）和细胞周期蛋白D1）的表达。
HPV阳性率为42%；几乎所有均为16型。HPV阳性与pRb和细胞周期蛋白D1表达降低、p16过表达以及患者年龄较轻之间存在统计学关联。p27下调的肿瘤往往pRb和p21也下调。
HPV阳性的扁桃体SCC具有独特的分子通路。它们与患者年龄较轻的关联表明，它们在生物学上与HPV阴性肿瘤不同。
