Yu Yue Yue, Dai Gu, Chen Jie, Wen Chuan Jun, Zhao Dong Hong, Li Chao Jun
Life Science School of Nanjing Normal University, Nanjing 210097.
Shi Yan Sheng Wu Xue Bao. 2003 Oct;36(5):335-41.
Calmodulin (CaM) is a major cytoplasmic Ca2+ receptor and performs a multiplicity of functions in the cell. By using GFP-CaM fusion protein, we have studied the detailed dynamic redistribution of CaM during cytokinesis in HeLa cells. CaM associates with midbody in late cytokinesis phase. When the cells were treated with Ca2+/CaM inhibitor W7, the dissolving of the midbody was delayed. Moreover, we have found that gamma-tubulin colocalized with CaM at the midbody during cytokinesis. W7 treatment could affect the dissociation of gamma-tubulin from midbody. These results suggest that CaM may involve in the regulation of midbody microtubules disassembly and may thus affect the completion of cytokinesis.
钙调蛋白（CaM）是一种主要的细胞质Ca2+受体，在细胞中发挥多种功能。通过使用绿色荧光蛋白（GFP）-CaM融合蛋白，我们研究了HeLa细胞胞质分裂过程中CaM详细的动态重新分布。CaM在胞质分裂后期与中间体结合。当细胞用Ca2+/CaM抑制剂W7处理时，中间体的溶解被延迟。此外，我们发现在胞质分裂期间，γ-微管蛋白与CaM在中间体处共定位。W7处理会影响γ-微管蛋白从中间体的解离。这些结果表明，CaM可能参与中间体微管拆卸的调节，从而可能影响胞质分裂的完成。
