Yu Yue Yue, Dai Gu, Pan Fei Yan, Chen Jie, Li Chao Jun
Jiangsu Key Laboratory for Molecular and Medical Biotechnology, School of Life Sciences, Nanjing Normal University. Nanjing 210097, China.
Cell Res. 2005 Jul;15(7):548-52. doi: 10.1038/sj.cr.7290324.
A transient postanaphase repositioning of the centriole is found to control the completion of cytokinesis. Using a green fluorescent protein-calmodulin fusion protein as a living cell probe, we have previously found that calmodulin is associated with the initiation and progression of cytokinesis. In this study, we further studied the effect of calmodulin on the repositioning of the centriole and subsequent cell cycle progression. When activity of calmodulin is inhibited, the regression of the centriole from the intercellular bridge to the cell center is blocked, and thus the completion of cell division is repressed and two daughter cells are linked by longer cell bridge in perturbed cells. W7 treatment during cytokinesis also results in unfinished cytokinesis and stopped G1 phase. These results suggest that calmodulin activity is required for centriole repositioning and can affect the completion of cytokinesis and cell cycle progression.
研究发现，中心粒在后期后的短暂重新定位可控制胞质分裂的完成。我们之前使用绿色荧光蛋白-钙调蛋白融合蛋白作为活细胞探针，发现钙调蛋白与胞质分裂的起始和进程相关。在本研究中，我们进一步研究了钙调蛋白对中心粒重新定位及随后细胞周期进程的影响。当钙调蛋白的活性受到抑制时，中心粒从细胞间桥向细胞中心的回归受阻，因此细胞分裂的完成受到抑制，在受干扰的细胞中，两个子细胞通过更长的细胞桥相连。在胞质分裂期间进行W7处理也会导致胞质分裂未完成以及G1期停滞。这些结果表明，钙调蛋白活性是中心粒重新定位所必需的，并且会影响胞质分裂的完成和细胞周期进程。
