Wang Li Lin, Pang Ji Liang, Hu Jiang Qin, Zhu Mu Yuan, Liang Hai Man
College of Life Sciences, Zhejiang University, School of Life Sciences, Hangzhou Normal College, Hangzhou 310036.
Shi Yan Sheng Wu Xue Bao. 2003 Oct;36(5):347-52.
Changes of endogenous hormones and polyamines(PAs) contents during floral differentiation (0-6 day) in cucumber cotyledonary nodes cultured in vitro were determined by using HPLC. The results showed that all four endogenous hormones decreased obviously within 0-2 day and increased slightly within 4-5 day after culture. This indicated that low levels of indole-3-acetic acid (IAA), gibberelline(GA3) and abscisin acid(ABA) during 0-2 day were favorable to floral primordia differentiation, while high level of zeatin(ZT) during 3-5 day was favorable to floral organ primordia differentiation. Contents of spermine(Spm), spermidine(Spd) and cadavarine(Cad) were decreased within 0-1 day, but increased within 1-4 day, and then decreased again within 4-5 day after culture. On the contrary, content of putrescine(Put) was increased significantly on the first day and then declined during 1-6 day. Changes of PAs contents indicated that high levels of PAs and Put were favorable to floral primordia differentiation, the increasing of Spm after the second day was favorable to floral organ primordia differentiation, and change of Cad content may be one of the characteristic symbols between floral and vegetative bud differentiation.
采用高效液相色谱法（HPLC）测定了离体培养的黄瓜子叶节在花芽分化期（0 - 6天）内内源激素和多胺（PAs）含量的变化。结果表明，培养后0 - 2天内所有4种内源激素均明显下降，4 - 5天略有上升。这表明0 - 2天内低水平的吲哚 - 3 - 乙酸（IAA）、赤霉素（GA3）和脱落酸（ABA）有利于花原基分化，而3 - 5天内高水平的玉米素（ZT）有利于花器官原基分化。精胺（Spm）、亚精胺（Spd）和尸胺（Cad）的含量在培养后0 - 1天下降，但在1 - 4天上升，然后在4 - 5天再次下降。相反，腐胺（Put）含量在第1天显著增加，然后在1 - 6天下降。多胺含量的变化表明，高水平的多胺和Put有利于花原基分化，第2天后Spm的增加有利于花器官原基分化，Cad含量的变化可能是花芽和叶芽分化之间的特征标志之一。
