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[高温胁迫下半夏珠芽倒苗前后内源激素的变化]

[Change of endogenous hormone around sprout tumble of Pinellia ternata under high temperature stress].

作者信息

Xue Jian-Ping, Zhang Ai-Min, Yang Jian, Chang Li, Huang Yue-Qin

机构信息

College of Food and Bioengineering, Zhengzhou University of Light Industry, Zhengzhou 450002, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2007 Dec;32(23):2489-91.

PMID:18330239
Abstract

OBJECTIVE

To study the change of endogenous hormone (ABA, IAA, JA, GA3, ZR) in the leaves, petioles, tubers of Pinellia ternate around sprout tumble. It also provided some valuable information to prevent sprout tumble and increase production.

METHOD

Tubers of P. ternata were cultured firstly at (23 +/- 1) degree C for certain days, and then they were coerced under (30 +/- 1 ) degree C stress in the same artificial climate boxes. The endogenous hormones in leaves, petioles and tubers during different stages of high temperature stress were determined with Enzyme-linked Immunosorbent Assays (ELISA).

RESULT

After under high temperature stress, ABA content in leaves, petioles and tubers increased obviously. Similarly, JA content rose all in the leaves, petioles and tubers. But in the same conditions IAA content declined significantly in the leaves and petioles. In the tubers, IAA content also decreased, but not quickly. With the extension of high temperature coercion, the leaves, petioles, tubers, ZR content were gradually falling off. In the leaves of GA3 content rose markedly at the third day, fell down at the sixth day, but remained higher than before treatment. With the extension of the processing time, GA3 content fell off in the petioles and tubers.

CONCLUSION

ABA, JA, ZT and GA3 played an important role in controlling sprout tumble of P. ternata.

摘要

目的

研究半夏在倒苗前后叶片、叶柄、块茎中内源激素(脱落酸、生长素、茉莉酸、赤霉素、玉米素核苷)的变化情况,为防止半夏倒苗、提高产量提供参考依据。

方法

将半夏块茎先在(23±1)℃培养一定天数,然后置于(30±1)℃的人工气候箱中进行高温胁迫处理。采用酶联免疫吸附测定法(ELISA)测定高温胁迫不同阶段叶片、叶柄和块茎中的内源激素含量。

结果

高温胁迫后,叶片、叶柄和块茎中的脱落酸含量明显增加。同样,茉莉酸含量在叶片、叶柄和块茎中均上升。但在相同条件下,叶片和叶柄中的生长素含量显著下降。在块茎中,生长素含量也下降,但下降速度较慢。随着高温胁迫时间的延长,叶片、叶柄、块茎中的玉米素核苷含量逐渐下降。叶片中的赤霉素含量在第三天显著上升,第六天下降,但仍高于处理前。随着处理时间的延长,叶柄和块茎中的赤霉素含量下降。

结论

脱落酸、茉莉酸、玉米素核苷和赤霉素在控制半夏倒苗过程中起重要作用。

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