Wang Li Lin, Pang Ji Liang, Hu Jiang Qin, Zhang Yan Yoan, Liang Hai Man
School of Life Sciences, Hangzhou Normal College, Hangzhou 310036.
Shi Yan Sheng Wu Xue Bao. 2002 Jun;35(2):147-50.
Cotyledonary nodes of cucumber cultured on calcium-free medium for 0, 1, 2, 3, 4, 5, 6d respectively, were transferred to medium with 6.0 mmol/L CaCl2 for 24h, then returned to calcium-free medium. Cotyledonary nodes cultured on calcium-free or 6.0 mmol/L CaCl2 medium for all time, were taken as controls. Results showed that cotyledonary nodes were transferred to 6.0 mmol/L CaCl2 medium for 24h during 0-3d after the beginning of culture, percentage of floral bud formation at cotyledonary nodes was increased significantly. Transferring cotyledonary nodes on the 3d day after the beginning of culture was achieved best effect, percentage of floral bud formation was up to 34.3%. We deduced that the calcium sensitive period during floral differentiation of cucumber cotyleddonary node cultured in vitro may be 0-4d after the beginning of culture.
将黄瓜子叶节分别在无钙培养基上培养0、1、2、3、4、5、6天，然后转移至含有6.0 mmol/L氯化钙的培养基中培养24小时，之后再放回无钙培养基。一直培养在无钙或6.0 mmol/L氯化钙培养基上的子叶节作为对照。结果表明，在培养开始后的0 - 3天内将子叶节转移至6.0 mmol/L氯化钙培养基中培养24小时，子叶节花芽形成率显著提高。在培养开始后第3天转移子叶节效果最佳，花芽形成率高达34.3%。我们推测，离体培养的黄瓜子叶节花芽分化过程中的钙敏感期可能是培养开始后的0 - 4天。
