Chalifa-Caspi Vered, Shmueli Orit, Benjamin-Rodrig Hila, Rosen Naomi, Shmoish Michael, Yanai Itai, Ophir Ron, Kats Pavel, Safran Marilyn, Lancet Doron
Bioinformatics Support Unit, Life Sciences Dept., Ben-Gurion University of the Negev, Beer-Sheva, Israel.
Brief Bioinform. 2003 Dec;4(4):349-60. doi: 10.1093/bib/4.4.349.
The interpretation of microarray expression results often includes extensive efforts to identify and annotate the gene representatives immobilised on the arrays. In this paper we describe the usage of our automatic GeneAnnot system, which links between Affymetrix arrays and the rich human gene annotations available in GeneCards. We explain GeneCards search options and results display; elaborate on the presentation of expression information in GeneCards, including both our whole-genome GeneNote project and external expression resources; describe the various parameters and displays used by GeneAnnot to assess the annotation quality and probeset specificity; and show how to search GeneAnnot and GeneNote websites directly.
微阵列表达结果的解读通常需要付出大量努力来识别和注释固定在阵列上的基因代表。在本文中，我们描述了我们的自动基因注释系统（GeneAnnot）的使用方法，该系统将Affymetrix阵列与GeneCards中丰富的人类基因注释联系起来。我们解释了GeneCards的搜索选项和结果显示；详细阐述了GeneCards中表达信息的呈现方式，包括我们的全基因组基因注释项目（GeneNote）和外部表达资源；描述了GeneAnnot用于评估注释质量和探针集特异性的各种参数和显示方式；并展示了如何直接搜索GeneAnnot和GeneNote网站。
