妊娠类天疱疮中的组织嗜酸性粒细胞增多：与嗜酸性粒细胞趋化因子及迁移的嗜酸性粒细胞上上调的活化标志物的关联
Tissue eosinophilia in pemphigoid gestationis: association with eotaxin and upregulated activation markers on transmigrated eosinophils.
作者信息
Günther Claudia, Wozel Gottfried, Dressler Jan, Meurer Michael, Pfeiffer Christiane
机构信息
Department of Dermatology, University Hospital Carl Gustav Carus at the Technical University of Dresden, Dresden, Germany.
出版信息
Am J Reprod Immunol. 2004 Jan;51(1):32-9. doi: 10.1046/j.8755-8920.2003.00118.x.
PROBLEM
We studied chemotactic cytokines and activation of skin-homed eosinophils in pemphigoid gestationis, a rare autoimmune bullous disease of late pregnancy.
METHOD OF STUDY
Eotaxin and interleukin (IL)-5 were analysed in patients' blister fluid and serum by enzyme-linked immunosorbent assay. Lesional tissue was investigated for eotaxin deposition by immunohistochemistry. Adhesion molecules and activation markers on tissue eosinophils were analysed by immunofluorescence staining.
RESULTS
Eotaxin was elevated in serum of two pemphigoid gestationis patients compared with nine healthy pregnant women. Blister fluid contained even higher amounts of eotaxin and in addition IL-5. Eotaxin was mainly expressed in subepidermal tissue in close proximity to eosinophils that expressed high levels of adhesion molecules (CD11b, CD11c, CD18 and CD49d) and the activation marker HLA-DR.
CONCLUSIONS
High local levels of eotaxin and IL-5 as present in the blister fluid correspond to the high state of activation of the infiltrated eosinophils.
问题
我们研究了妊娠类天疱疮（一种罕见的妊娠晚期自身免疫性大疱性疾病）中趋化性细胞因子及皮肤定居嗜酸性粒细胞的活化情况。
研究方法
采用酶联免疫吸附测定法分析患者水疱液和血清中的嗜酸性粒细胞趋化因子及白细胞介素（IL）-5。通过免疫组织化学研究病变组织中嗜酸性粒细胞趋化因子的沉积情况。采用免疫荧光染色分析组织嗜酸性粒细胞上的黏附分子和活化标志物。
结果
与9名健康孕妇相比，两名妊娠类天疱疮患者血清中的嗜酸性粒细胞趋化因子水平升高。水疱液中嗜酸性粒细胞趋化因子含量更高，此外还含有IL-5。嗜酸性粒细胞趋化因子主要表达于紧邻表达高水平黏附分子（CD11b、CD11c、CD18和CD49d）及活化标志物HLA-DR的嗜酸性粒细胞的表皮下组织中。
结论
水疱液中存在的高局部水平的嗜酸性粒细胞趋化因子和IL-5与浸润嗜酸性粒细胞的高活化状态相对应。
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