Hammadeh Mohamad Eid, Fischer-Hammadeh Constanze, Hoffmeister Heidrun, Herrmann Wolfgang, Rosenbaum Peter, Schmidt Werner
Department of Obstetrics and Gynecology, The University of Saarland, Homburg/Saar, Germany.
Am J Reprod Immunol. 2004 Jan;51(1):81-5. doi: 10.1046/j.8755-8920.2003.00121.x.
The aim of this study was (i) to investigate the existence of fibroblast growth factor (FGF), soluble intracellular adhesion molecule-1 (sICAM-1), and stem cell factor (SCF) in serum and human follicular fluid (FF) of intracytoplasmic sperm injection (ICSI) patients, and (ii) to determine the relationship between these parameters and ICSI outcome.
Seventy-five patients undergoing controlled ovarian hyperstimulation with human menopausal gonadotropin (hMG) after down-regulation with GnRHa were included in this study. The concentrations of FGF, SCF, and sICAM-1 were measured by using commercially available enzyme-linked immunosorbent assay test kits.
The FGF, sICAM-1, and SCF concentrations in the serum of women who become pregnant (group I) were 8.5 +/- 1.5 pg/mL, 235.8 +/- 81.1 ng/mL, and 597.7 +/- 139.9 pg/mL, and the corresponding concentrations of women who did not (group II) were 6.4 +/- 3.6 pg/mL, 230.6 +/- 66.5 ng/mL, and 569.6 +/- 91.4 pg/mL respectively. No significant difference was observed between the two investigated groups with regard to the number of hMG ampoules administered for controlled ovarian hyperstimulation, estradiol concentration on the day of human chorionic gonadotropin (hCG) injection, number of retrieved oocytes and fertilization rate.
The concentration of FGF, sICAM-1, and SCF did not differ significantly between the two groups in serum or in FF. Besides, the ICSI outcome was not related to their concentrations in serum or FF. Therefore, these parameters could not be used as a prognostic factor in ICSI program.
本研究的目的是（i）调查胞浆内单精子注射（ICSI）患者血清和人卵泡液（FF）中成纤维细胞生长因子（FGF）、可溶性细胞间黏附分子-1（sICAM-1）和干细胞因子（SCF）的存在情况，以及（ii）确定这些参数与ICSI结果之间的关系。
本研究纳入了75例在使用促性腺激素释放激素激动剂（GnRHa）进行降调节后接受人绝经期促性腺激素（hMG）控制性卵巢过度刺激的患者。使用市售的酶联免疫吸附测定试剂盒测量FGF、SCF和sICAM-1的浓度。
怀孕女性（第一组）血清中的FGF、sICAM-1和SCF浓度分别为8.5±1.5 pg/mL、235.8±81.1 ng/mL和597.7±139.9 pg/mL，未怀孕女性（第二组）的相应浓度分别为6.4±3.6 pg/mL、230.6±66.5 ng/mL和569.6±91.4 pg/mL。在用于控制性卵巢过度刺激的hMG安瓿数量、人绒毛膜促性腺激素（hCG）注射日的雌二醇浓度、回收的卵母细胞数量和受精率方面，两个研究组之间未观察到显著差异。
两组血清或FF中的FGF、sICAM-1和SCF浓度无显著差异。此外，ICSI结果与它们在血清或FF中的浓度无关。因此，这些参数不能用作ICSI程序中的预后因素。
