Unit of Endocrinology, Diabetes Mellitus and Metabolism, Aretaieion Hospital, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
Faculty of Animal Science and Aquaculture, Department of Anatomy and Physiology of Farm Animals, Agricultural University of Athens, Athens, Greece.
J Assist Reprod Genet. 2018 Jun;35(6):1019-1025. doi: 10.1007/s10815-018-1170-0. Epub 2018 Apr 4.
To calculate the concentrations of interleukin 15 (IL-15) in follicular fluid (FF) and evaluate their relation with oocyte maturation, follicle size, and patients' body mass index (BMI) and age.
Follicular fluid specimens were obtained from 56 subfertile women undergoing intracytoplasmic sperm injection (ICSI) during oocyte retrieval for measurement of IL-15 concentrations with ELISA. Wilcoxon's test and Pearson's correlation coefficient were used to correlate FF concentrations of IL-15 with follicular size and stage of oocyte maturation, along with patients' BMI and age.
IL-15 concentrations in FF of follicles with immature oocytes were significantly greater than those from follicles with mature ones (median 5.333 vs. 3.250 pg/ml, respectively, p < 0.001). There was a significant negative correlation between IL-15 concentrations and follicle size (r = - 0.333, p = 0.003). No significant correlation was observed between IL-15 concentrations and patients' BMI and age (p > 0.05).
IL-15 concentrations in FF are adversely related with the size of the follicles and the maturity of the corresponding retrieved oocytes in a cohort of expected normal responders undergoing intracytoplasmic sperm injection (ICSI). Follicular fluid concentrations of IL-15 should be investigated as a possible predictive factor for oocyte maturity.
计算卵泡液（FF）中白细胞介素 15（IL-15）的浓度，并评估其与卵母细胞成熟、卵泡大小以及患者的体重指数（BMI）和年龄的关系。
从 56 名接受卵胞浆内单精子注射（ICSI）的不孕妇女的卵泡液中提取样本，用 ELISA 法测量 IL-15 的浓度。采用 Wilcoxon 检验和 Pearson 相关系数来分析 IL-15 在 FF 中的浓度与卵泡大小和卵母细胞成熟阶段以及患者的 BMI 和年龄之间的相关性。
不成熟卵母细胞的卵泡液中 IL-15 的浓度明显高于成熟卵母细胞的卵泡液（中位数分别为 5.333 pg/ml 和 3.250 pg/ml，p<0.001）。IL-15 浓度与卵泡大小呈显著负相关（r=-0.333，p=0.003）。IL-15 浓度与患者的 BMI 和年龄之间无显著相关性（p>0.05）。
在接受 ICSI 的预期正常反应者中，FF 中 IL-15 的浓度与卵泡大小和相应取回的卵母细胞的成熟度呈负相关。卵泡液中 IL-15 的浓度应作为卵母细胞成熟的可能预测因子进行研究。
