激光极化129Xe在生物分子分析中的应用。

Applications of laser-polarized 129Xe to biomolecular assays.

作者信息

Lowery Thomas J, Rubin Seth M, Ruiz E Janette, Spence Megan M, Winssinger Nicolas, Schultz Peter G, Pines Alexander, Wemmer David E

机构信息

Department of Chemistry, University of California at Berkeley, Berkeley, CA 94720, USA.

出版信息

Magn Reson Imaging. 2003 Dec;21(10):1235-9. doi: 10.1016/j.mri.2003.08.025.

DOI:10.1016/j.mri.2003.08.025

PMID:14725931

Abstract

The chemical shift sensitivity and significant signal enhancement afforded by laser-polarized 129Xe have motivated the application of 129Xe NMR to biological imaging and spectroscopy. Recent research done by our group has used laser-polarized 129Xe in biomolecular assays that detect ligand-binding events and distinguish protein conformations. The successful application of unfunctionalized and functionalized 129Xe NMR to in vitro biomolecular assays suggests the potential future use of a functionalized xenon biosensor for in vivo imaging.

摘要

激光极化的¹²⁹Xe所提供的化学位移敏感性和显著的信号增强推动了¹²⁹Xe NMR在生物成像和光谱学中的应用。我们小组最近的研究已将激光极化的¹²⁹Xe用于检测配体结合事件和区分蛋白质构象的生物分子分析中。未功能化和功能化的¹²⁹Xe NMR在体外生物分子分析中的成功应用表明，功能化氙生物传感器未来有可能用于体内成像。