Panayiotidis Mihalis I, Stabler Sally P, Allen Robert H, Ahmad Aftab, White Carl W
Department of Pediatrics, National Jewish Medical and Research Center, 1400 Jackson Street, Denver, CO 80206, USA.
Chem Biol Interact. 2004 Jan 15;147(1):87-97. doi: 10.1016/j.cbi.2003.10.009.
The effect of cigarette smoke extract (CSE) on S-adenosylmethionine (SAM), S-adenosylhomocysteine (SAH), and sulfur amino acid metabolism was examined in human lung epithelial-like (A549) cells exposed to various CSE concentrations (2.5-100%) for 24 or 48 h. Intracellular SAM and SAM/SAH ratio were elevated after exposure to CSE for 48 h. Cell SAH content decreased, but the effect was not consistent. Cellular cystathionine, cysteine, and methionine levels were increased after CSE exposure for 48h. Sub-acute exposure to CSE induced increases in cellular SAM and SAM/SAH ratio. The transsulfuration pathway was likely activated by CSE since cystathionine increased, potentially contributing to the increased total intracellular GSH content.
在暴露于不同浓度（2.5%-100%）香烟烟雾提取物（CSE）24或48小时的人肺上皮样（A549）细胞中，检测了CSE对S-腺苷甲硫氨酸（SAM）、S-腺苷同型半胱氨酸（SAH）和含硫氨基酸代谢的影响。暴露于CSE 48小时后，细胞内SAM和SAM/SAH比值升高。细胞SAH含量降低，但效果不一致。暴露于CSE 48小时后，细胞内胱硫醚、半胱氨酸和甲硫氨酸水平升高。亚急性暴露于CSE可导致细胞内SAM和SAM/SAH比值升高。由于胱硫醚增加，转硫途径可能被CSE激活，这可能导致细胞内总谷胱甘肽（GSH）含量增加。
