Zhang Lisheng, Freedman Neil J, Brian Leigh, Peppel Karsten
Duke University Department of Medicine (Cardiology), Duke University Medical Center, Durham, NC 27710, USA.
Arterioscler Thromb Vasc Biol. 2004 Mar;24(3):470-6. doi: 10.1161/01.ATV.0000116865.98067.31. Epub 2004 Jan 15.
Vein graft disease involves neointimal smooth muscle cells, the origins of which are unclear. This study sought to characterize and quantitate vein graft infiltration by cells extrinsic to the graft in a mouse model of vein graft disease.
Inferior vena cava-to-carotid artery interposition grafting between C57Bl/6 and congenic beta-galactosidase-expressing ROSA26 mice was performed. Vein grafts were harvested 6 weeks postoperatively and stained with X-gal. More than 60% of neointimal cells derived from the recipient, and 50% of these cells expressed smooth muscle alpha-actin. The distribution of donor and recipient-derived cells within this vein graft wall layer was distinctly focal, consistent with focal infiltration and expansion of progenitor cells. When bone marrow transplantation with congenic green fluorescent protein (GFP)-expressing cells was used in vein graft recipients 1 month before surgery, abundant GFP-expressing cells appeared in the media, but not the neointima, of mature grafts. Endothelial cells in mature grafts derived from graft-intrinsic and graft-extrinsic sources and were, in part, of bone marrow origin.
Cells extrinsic to the graft, including bone marrow-derived cells, predominate during vein graft remodeling.
静脉移植物病变涉及新生内膜平滑肌细胞，但其来源尚不清楚。本研究旨在在静脉移植物病变小鼠模型中，对移植物外源性细胞浸润静脉移植物的情况进行特征描述和定量分析。
在C57Bl/6小鼠和表达β-半乳糖苷酶的同基因ROSA26小鼠之间进行下腔静脉至颈动脉的间置移植。术后6周采集静脉移植物并进行X-gal染色。超过60%的新生内膜细胞来源于受体，其中50%的细胞表达平滑肌α-肌动蛋白。供体和受体来源的细胞在该静脉移植物壁层内的分布明显呈局灶性，与祖细胞的局灶性浸润和扩张一致。当在手术前1个月对静脉移植物受体进行同基因表达绿色荧光蛋白（GFP）细胞的骨髓移植时，在成熟移植物的中膜而非新生内膜中出现了大量表达GFP的细胞。成熟移植物中的内皮细胞来源于移植物内源性和移植物外源性，部分为骨髓来源。
包括骨髓来源细胞在内的移植物外源性细胞在静脉移植物重塑过程中占主导地位。
