Chen Jianjun, Devanand Pachanoor S, Norman David J, Henny Richard J, Chao Chih-cheng T
Mid-Florida Research and Education Center, and Department of Environmental Horticulture, University of Florida, 2725 Binion Road, Apopka, FL 32703-8504, USA.
Ann Bot. 2004 Feb;93(2):157-66. doi: 10.1093/aob/mch025.
Aglaonema is an important ornamental foliage plant genus, but genetic relationships among its species and cultivars have not been reported. This study analysed genetic relatedness of 54 cultivars derived from nine species using amplified fragment length polymorphism (AFLP) markers.
Initially, 48 EcoRI + 2/MseI + 3 primer set combinations were screened, from which six primer sets that showed clear scoreable and highly polymorphic fragments were selected and used for AFLP reactions. AFLP fragments were scored and entered into a binary data matrix as discrete variables. Jaccard's coefficient of similarity was calculated for all pair-wise comparisons among the 54 cultivars, and a dendrogram was constructed by the unweighted pair-group method using the arithmetic average (UPGMA).
The number of AFLP fragments generated per primer set ranged from 59 to 112 with fragment sizes varying from 50 to 565 bp. A total of 449 AFLP fragments was detected, of which 314 were polymorphic (70 %). All cultivars were clearly differentiated by their AFLP fingerprints. The 54 cultivars were divided into seven clusters; cultivars within each cluster generally share similar morphological characteristics. Cluster I contains 35 cultivars, most of them are interspecific hybrids developed mainly from A. commutatum, A. crispum or A. nitidum. However, Jaccard's similarity coefficients among these hybrids are 0.84 or higher, suggesting that these popular hybrid cultivars are genetically much closer than previously thought. This genetic similarity may imply that A. nitidum and A. crispum are likely progenitors of A. commutatum.
Results of this study demonstrate the efficiency and ease of using AFLP markers for investigating genetic relationships of ornamental foliage plants, a group usually propagated vegetatively. The AFLP markers developed will help future Aglaonema cultivar identification, germplasm conservation and new cultivar development.
广东万年青是一种重要的观叶植物属，但尚未见有关其种间和品种间亲缘关系的报道。本研究利用扩增片段长度多态性（AFLP）标记分析了9个种的54个品种的遗传相关性。
首先，对48种EcoRI + 2/MseI + 3引物组合进行筛选，从中选出6种能产生清晰可计分且多态性高的片段的引物组合用于AFLP反应。对AFLP片段进行计分，并作为离散变量输入二元数据矩阵。计算54个品种间所有两两比较的杰卡德相似系数，并采用非加权组平均法（UPGMA）构建聚类图。
每个引物组合产生的AFLP片段数量在59至112之间，片段大小在50至565 bp之间。共检测到449个AFLP片段，其中314个具有多态性（70%）。所有品种都通过其AFLP指纹图谱得到了清晰区分。54个品种被分为7个类群；每个类群内的品种通常具有相似的形态特征。第一类群包含35个品种，其中大多数是主要由银皇后、皱叶亮丝草或亮丝草培育出的种间杂种。然而，这些杂种之间的杰卡德相似系数为0.84或更高，表明这些受欢迎的杂交品种在遗传上比之前认为的更为接近。这种遗传相似性可能意味着亮丝草和皱叶亮丝草可能是银皇后的祖先。
本研究结果证明了利用AFLP标记研究观叶植物遗传关系的有效性和简便性，观叶植物通常通过无性繁殖。所开发的AFLP标记将有助于未来广东万年青品种鉴定、种质保存和新品种培育。
