Nasrolahei Mohtatam, Maleki Iraj, Emadian Omid
Microbiology and Immunology Department, Sari Medical School, Iran.
Rom J Gastroenterol. 2003 Dec;12(4):293-6.
With regard to the presence of Helicobacter pylori (H.pylori ) in dental plaque and its association with gastric infection, data from the literature are controversial. The aim of this study was to determine the prevalence of H.pylori in dental plaque of dyspeptic patients who underwent upper gastrointestinal endoscopy and to investigate its relationship to gastric infection.
In a cross sectional study, over one year ( February 2002-February 2003) samples were taken with separate sterile curretes from tooth surfaces of molars, premolars and incisors of 180 consecutive dyspeptic patients referred to the Endoscopy Department of Emmam Khomeini Hospital. Dental plaque samples were subjected to the rapid urease test, Giemsa staining, PCR test and culture for identification of H.pylori. During endoscopy, six biopsies were taken from the gastric antrum and corpus and histological examination, Giemsa stainings, Rapid Urease test and culture were performed. Patients were defined as infected when both rapid urease test and Giemsa stain, or when cultures were positive for H.pylori. Information concerning oral hygiene protocol were recorded. For data analysis, the chi square test was used and P < 0.05 was defined statistically significant.
Our data proved the presence of H. pylori in dental plaque of infected and uninfected patients. There was no significant association between H. pylori colonization in dental plaque and gastric infection. In this study, oral hygiene (the frequency of dental visits and teeth cleaning) did not have a significant influence on the presence of H. pylori in dental plaque.
Dental plaque is the reservoir of H.pylori with no relationship to gastric infection.
关于牙菌斑中幽门螺杆菌（H.pylori）的存在及其与胃部感染的关联，文献数据存在争议。本研究的目的是确定接受上消化道内镜检查的消化不良患者牙菌斑中幽门螺杆菌的患病率，并研究其与胃部感染的关系。
在一项横断面研究中，在一年多的时间里（2002年2月至2003年2月），从连续转诊至伊玛目霍梅尼医院内镜科的180名消化不良患者的磨牙、前磨牙和切牙的牙齿表面，用单独的无菌刮匙采集样本。牙菌斑样本进行快速尿素酶试验、吉姆萨染色、聚合酶链反应（PCR）试验和培养以鉴定幽门螺杆菌。在内镜检查期间，从胃窦和胃体取六块活检组织，进行组织学检查、吉姆萨染色、快速尿素酶试验和培养。当快速尿素酶试验和吉姆萨染色均为阳性，或培养出幽门螺杆菌时，患者被定义为感染。记录有关口腔卫生方案的信息。数据分析采用卡方检验，P<0.05被定义为具有统计学意义。
我们的数据证明感染和未感染患者的牙菌斑中均存在幽门螺杆菌。牙菌斑中幽门螺杆菌定植与胃部感染之间无显著关联。在本研究中，口腔卫生（看牙频率和牙齿清洁情况）对牙菌斑中幽门螺杆菌的存在没有显著影响。
牙菌斑是幽门螺杆菌的储存库，与胃部感染无关。
