Zhang Jiang-Ping, Peng Zhen-Hui, Zhang Ju, Zhang Xiang-Hong, Zheng Qing Yin
Department of Otolaryngology, Second Hospital of Xi'an Jiaotong University, Shaanxi Province, China.
World J Gastroenterol. 2006 Jan 21;12(3):468-72. doi: 10.3748/wjg.v12.i3.468.
To detect whether there is Helicobacter pylori (H pylori) colonization in the pharynx mucous membrane of healthy people and whether chronic pharyngitis is related to H pylori infection.
Fifty cases of chronic pharyngitis refractory over three months were prospectively studied from March 2004 to August 2004 in the otolaryngology outpatient department of the Second Hospital of Xi'an Jiaotong University. Template-directed dye-terminator incorporated with fluorescence polarization detection (TDI-FP) and modified Giemsa stain were used to examine pharynx mucous membrane tissue for H pylori colonization in the patients with chronic pharyngitis and the healthy people as a control group.
In the control group, no people were detected to have H pylori in the pharynx. In contrast, in 50 cases with chronic pharyngitis, 19 (38.0%) cases were H pylori positive with a TDI-FP assay and 4 (8%) cases were TDI-FP positive with Giemsa staining in the pharynx. Sixteen of the 50 pharyngitis cases had stomach ailment history, 11 cases (68.8%) of these 16 patients were determined to be H pylori positive in the pharynx with the TDI-FP assay. c2 test showed that this infection rate was remarkably higher (P=0.0007) than that in the cases without stomach ailment history. Giemsa staining showed that 3 cases (18.8%) of the patients with stomach ailment history were infected with H pylori in the pharynx, which was remarkably higher (P=0.042) than that in the patients without stomach ailment history (1 case, which was 2.9%).
H pylori may not be detected in the pharynx of healthy people. Chronic pharyngitis may be related to H pylori infection. The infection rate with H pylori in the pharynx is higher in patients with stomach ailment histories than in patients without stomach ailment histories, suggesting that chronic pharyngitis may be related to stomach ailment history.
检测健康人咽黏膜中是否存在幽门螺杆菌（H pylori）定植，以及慢性咽炎是否与H pylori感染相关。
2004年3月至2004年8月，在西安交通大学第二医院耳鼻喉科门诊对50例病程超过3个月的慢性咽炎难治性患者进行前瞻性研究。采用模板指导的染料终止剂结合荧光偏振检测（TDI-FP）和改良吉姆萨染色法，对慢性咽炎患者及作为对照组的健康人的咽黏膜组织进行H pylori定植检测。
对照组中，未检测到咽部有H pylori的人。相比之下，50例慢性咽炎患者中，TDI-FP检测法显示19例（38.0%）咽部H pylori阳性，吉姆萨染色法显示4例（8%）咽部TDI-FP阳性。50例咽炎患者中有16例有胃部疾病史，这16例患者中11例（68.8%）经TDI-FP检测法确定咽部H pylori阳性。卡方检验显示，该感染率显著高于（P = 0.0007）无胃部疾病史的患者。吉姆萨染色显示，有胃部疾病史的患者中有3例（18.
