Radwan A I, Bekairi S I, Mukayel A A
Animal Production and Health Section, Ministry of Agriculture and Water, Riyadh, Saudi Arabia.
Rev Sci Tech. 1992 Sep;11(3):845-57. doi: 10.20506/rst.11.3.630.
Six treatment regimens using oxytetracycline (OTC) combined with streptomycin (ST) were evaluated for eliminating Brucella melitensis from 480 naturally-infected sheep and goats. Cessation of shedding Brucella from udder secretions and absence of Brucella in selected tissues at autopsy were considered criteria for successful treatment. Four regimens were equally effective in eliminating Brucella in the treated groups of sheep and goats regardless of the source of antibiotics used. These were regimen A (OTC 20 mg/kg intravenously daily for 6 weeks, combined with ST 20 mg/kg intramuscularly [i.m.] daily for 3 weeks), regimen B (long-acting [LA]-OTC 20 mg/kg i.m. every 3 days for 6 weeks, with ST 20 mg/kg i.m. every 3 days for 3 weeks), regimens D and E (LA-OTC 28 mg/kg i.m. every 3 days for 6 weeks, with ST 20 mg/kg i.m. every 3 days for 3 weeks). However, regimen C (LA-OTC 20 mg/kg i.m. every 3 days for 6 weeks, with ST 20 mg/kg i.m. every 3 days for 3 weeks) eliminated Brucella in only 75 of 80 (94%) sheep and goats. Regimen F (LA-OTC 25 mg/kg i.m. every 2 days for 4 weeks, combined with ST 20 mg/kg i.m. every 2 days for 2 weeks) was the most practical, effective and least expensive regimen for eliminating Brucella in the 80 treated sheep and goats. Brucella melitensis biovar 2 was repeatedly isolated from the mammary secretions of all sheep and goats before treatment. It was also isolated repeatedly from the udder secretions of all non-treated control animals and from selected tissue specimens collected from the controls at necropsy.
评估了六种使用土霉素（OTC）联合链霉素（ST）的治疗方案，用于清除480只自然感染布鲁氏菌的绵羊和山羊体内的羊种布鲁氏菌。停止从乳腺分泌物中排出布鲁氏菌以及尸检时选定组织中不存在布鲁氏菌被视为治疗成功的标准。无论使用何种抗生素来源，四种方案在清除绵羊和山羊治疗组中的布鲁氏菌方面同样有效。这些方案是方案A（OTC 20毫克/千克静脉注射，每日一次，共6周，联合ST 20毫克/千克肌肉注射，每日一次，共3周），方案B（长效[LA]-OTC 20毫克/千克肌肉注射，每3天一次，共6周，ST 20毫克/千克肌肉注射，每3天一次，共3周），方案D和方案E（LA-OTC 28毫克/千克肌肉注射，每3天一次，共6周，ST 20毫克/千克肌肉注射，每3天一次，共3周）。然而，方案C（LA-OTC 20毫克/千克肌肉注射，每3天一次，共6周，ST 20毫克/千克肌肉注射，每3天一次，共3周）仅在80只绵羊和山羊中的75只（94%）体内清除了布鲁氏菌。方案F（LA-OTC 25毫克/千克肌肉注射，每2天一次，共4周，联合ST 20毫克/千克肌肉注射，每2天一次，共2周）是在80只治疗的绵羊和山羊中清除布鲁氏菌最实用、最有效且成本最低的方案。在治疗前，所有绵羊和山羊的乳腺分泌物中均反复分离出羊种布鲁氏菌生物变种2。在所有未治疗的对照动物的乳腺分泌物以及尸检时从对照动物采集的选定组织标本中也反复分离出该菌。
