Russo Ana M, Mancebo Orlando A, Monzón Carlos M, Gait Juan J, Casco Rubén D, Torioni de Echaide Susana M
Centro de Investigaciones y Transferencia-CIT Formosa (CONICET-Universidad Nacional de Formosa-Gobierno de la Provincia de Formosa), Formosa, Argentina.
Centro de Investigaciones y Transferencia-CIT Formosa (CONICET-Universidad Nacional de Formosa-Gobierno de la Provincia de Formosa), Formosa, Argentina.
Rev Argent Microbiol. 2016 Apr-Jun;48(2):147-53. doi: 10.1016/j.ram.2015.10.005. Epub 2016 Mar 9.
An epidemiological study of brucellosis was carried out in 516 goats and mixed flocks (goat/sheep) from the three agro-ecological regions of Formosa province, Argentina. Serum samples from a total of 25401 goats and 2453 sheeps were analyzed using buffered plate agglutination test (BPAT) and complement fixation test (CFT). Bacteriological and PCR analyses on milk samples from goats in three flocks with a history of brucellosis and recent abortions were also performed. Brucellosis was detected in four of the nine departments of the province with an overall prevalence of 2% and an intra-flock prevalence ranging between 1% and 40%. The proportion of infected flocks was 3.6%, 12% and 36% for the eastern, central and western regions, respectively. Brucella melitensis bv. 1 was isolated efrom goats for the first time in the province. The expected fragments of 827bp from the omp2ab gene (Brucella spp.) and 731bp from the insert IS711 (B. melitensis) were amplified by PCR. Detection of antibodies by BPAT and FCT in sheep cohabiting with goats suggests that infections could have been caused by B. melitensis, posing an additional risk to public health. Control and eradication programs for brucellosis should consider mixed flocks as a single epidemiological unit. The results indicate that brucellosis by B. melitensis bv1 is highly endemic in the central and western regions of Formosa province.
在阿根廷福尔摩沙省的三个农业生态区，对516只山羊及混合羊群（山羊/绵羊）进行了布鲁氏菌病的流行病学研究。使用缓冲平板凝集试验（BPAT）和补体结合试验（CFT）对总共25401只山羊和2453只绵羊的血清样本进行了分析。还对来自三个有布鲁氏菌病病史和近期流产史的羊群的山羊奶样进行了细菌学和PCR分析。在该省九个部门中的四个部门检测到了布鲁氏菌病，总体患病率为2%，群内患病率在1%至40%之间。东部、中部和西部地区受感染羊群的比例分别为3.6%、12%和36%。在该省首次从山羊中分离出羊种布鲁氏菌生物变种1。通过PCR扩增出了来自omp2ab基因（布鲁氏菌属）的827bp预期片段和来自插入序列IS711（羊种布鲁氏菌）的731bp预期片段。通过BPAT和FCT对与山羊同居的绵羊进行抗体检测表明，感染可能是由羊种布鲁氏菌引起的，对公共卫生构成了额外风险。布鲁氏菌病的控制和根除计划应将混合羊群视为一个单一的流行病学单位。结果表明，羊种布鲁氏菌生物变种1引起的布鲁氏菌病在福尔摩沙省的中部和西部地区高度流行。
