表面增强拉曼光谱作为探测细菌中特定生化成分的工具。
Surface-enhanced Raman spectroscopy as a tool for probing specific biochemical components in bacteria.
作者信息
Zeiri L, Bronk B V, Shabtai Y, Eichler J, Efrima S
机构信息
Department of Chemistry, Ben-Gurion University of the Negev, PO Box 653, Beer-Sheva, Israel 84105.
出版信息
Appl Spectrosc. 2004 Jan;58(1):33-40. doi: 10.1366/000370204322729441.
Treatment of bacteria with silver yields intense and highly specific surface-enhanced Raman spectroscopy (SERS) spectra from various cellular chemical components located in the vicinity of the silver colloids. In particular, we demonstrate an extreme sensitivity to flavin components associated with the cell envelope and to their state of oxidation. Different spectra, possibly associated with DNA, carboxylates, and perhaps phosphates, are obtained from the soluble interior fraction of the cell.
用银处理细菌会产生来自位于银胶体附近的各种细胞化学成分的强烈且高度特异性的表面增强拉曼光谱（SERS）光谱。特别是，我们证明了对与细胞膜相关的黄素成分及其氧化状态具有极高的灵敏度。从细胞的可溶性内部组分中获得了可能与DNA、羧酸盐以及或许还有磷酸盐相关的不同光谱。
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