National Engineering Laboratory for AIDS Vaccine, School of Life Science, Jilin University, Changchun 130012, China.
State Key Laboratory of Supramolecular Structure and Materials, College of Chemistry, Jilin University, Changchun 130012, China.
Int J Mol Sci. 2022 Nov 10;23(22):13868. doi: 10.3390/ijms232213868.
Surface-enhanced Raman spectroscopy (SERS) is powerful for structural characterization of biomolecules under physiological condition. Owing to its high sensitivity and selectivity, SERS is useful for probing intrinsic structural information of proteins and is attracting increasing attention in biophysics, bioanalytical chemistry, and biomedicine. This review starts with a brief introduction of SERS theories and SERS methodology of protein structural characterization. SERS-active materials, related synthetic approaches, and strategies for protein-material assemblies are outlined and discussed, followed by detailed discussion of SERS spectroscopy of proteins with and without cofactors. Recent applications and advances of protein SERS in biomarker detection, cell analysis, and pathogen discrimination are then highlighted, and the spectral reproducibility and limitations are critically discussed. The review ends with a conclusion and a discussion of current challenges and perspectives of promising directions.
表面增强拉曼光谱（SERS）在生理条件下对生物分子的结构特征分析非常有用。由于其具有高灵敏度和选择性，SERS 可用于探测蛋白质的固有结构信息，在生物物理学、生物分析化学和生物医学领域受到越来越多的关注。本文简要介绍了 SERS 理论和 SERS 方法在蛋白质结构特征分析中的应用。概述并讨论了 SERS 活性材料、相关的合成方法以及蛋白质-材料组装的策略，随后详细讨论了有辅助因子和无辅助因子的蛋白质的 SERS 光谱。接着重点介绍了蛋白质 SERS 在生物标志物检测、细胞分析和病原体鉴别中的应用和进展，并对光谱重现性和局限性进行了批判性讨论。最后总结了当前面临的挑战和有前景的研究方向。
