Zhu Tao, Yao Zhi, Yuan Han-na, Lu Bo-gang, Yang Shu-yuan
Department of Neurosurgery, General Hospital, Tianjin Medical University, Tianjin 300052, China.
Chin J Traumatol. 2004 Feb;7(1):32-5.
To study the changes of interleukin-1 beta (IL-1beta), tumor necrosis factor alpha (TNFalpha) and interleukin-6 (IL-6) levels in brain and plasma after brain injury and to assess the relationship between the cytokine levels and injury severity in rats.
A total of 51 male Wistar rats, weighing 280-340 g, were anesthetized with chloral hydrate (400 mg/kg body weight) through intraperitoneal injection and fixed on a stereotaxic instrument. Severe brain injury was created in 16 rats (severe injury group) and moderate brain injury in 18 rats (moderate injury group) by a fluid percussion model, and cytokine levels of IL-1beta, TNFalpha and IL-6 were measured with biological assay. And sham operation was made on the other 17 rats (control group).
In the control group, the levels of IL-1beta, TNFalpha and IL-6 were hardly detected in the cortex of the rats, but in the ipsilateral cortex of the rats in both injury groups, they increased obviously at 8 hours after injury. The increasing degree of these cytokines had no significant difference between the two injury groups. The levels of IL-6 in the plasma of all the rats increased slightly, whereas the levels of IL-1beta and TNFalpha were undetectable.
The increase of IL-1beta, TNFalpha and IL-6 levels is closely related to brain injury. The increased cytokine levels in the central nervous system are not parallel to those in the peripheral blood. It suggests that inflammatory cytokines play important roles in the secondary neural damage after brain injury.
研究脑损伤后大鼠脑内及血浆中白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNFα）和白细胞介素-6（IL-6）水平的变化，并评估细胞因子水平与损伤严重程度之间的关系。
选取51只体重280 - 340 g的雄性Wistar大鼠，经腹腔注射水合氯醛（400 mg/kg体重）麻醉后固定于立体定位仪上。采用液压冲击模型对16只大鼠造成重度脑损伤（重度损伤组），对18只大鼠造成中度脑损伤（中度损伤组），并通过生物学检测法测定IL-1β、TNFα和IL-6的细胞因子水平。另外17只大鼠进行假手术（对照组）。
对照组大鼠皮质中几乎检测不到IL-1β、TNFα和IL-6水平，但在两个损伤组大鼠的同侧皮质中，损伤后8小时这些因子水平明显升高。这两种损伤组之间这些细胞因子的升高程度无显著差异。所有大鼠血浆中IL-6水平略有升高，而IL-1β和TNFα水平未检测到。
IL-1β、TNFα和IL-6水平的升高与脑损伤密切相关。中枢神经系统中细胞因子水平的升高与外周血中的水平不平行。这表明炎性细胞因子在脑损伤后的继发性神经损伤中起重要作用。
