Agay Diane, Andriollo-Sanchez Maud, Claeyssen Richard, Touvard Laurence, Denis Josiane, Roussel Anne-Marie, Chancerelle Yves
Centre de recherches du service de santé des Armées, 24 Avenue des Maquis du Grésivaudan, BP87, 38702 La Tronche cedex, France.
Eur Cytokine Netw. 2008 Mar;19(1):1-7. doi: 10.1684/ecn.2008.0113. Epub 2008 Feb 26.
Previous studies have demonstrated the early appearance of inflammatory cytokines in the systemic circulation after thermal injury both in humans and animals. The aim of this study was to evaluate the time course of several cytokines, IL-6, TNF-alpha and IL-1beta in serum, lung, liver and brain of severely burned rats during the first week after thermal injury. Cytokine measurements were performed by enzyme-linked immunosorbent assay (ELISA). The comparison between the sham-burned animals and animals with third-degree burns on 20% or 40% of their total body surface area allowed for the study of the inflammatory process relative to the size of the injury. Serum IL-6 levels, which were undetectable in sham-treated animals, peaked during the first hours after injury and were proportionate to the size of the area burned. After a few days, IL-6 increased once more, but only in the most severely burned rats. In lung, liver and brain, low but measurable basal levels of TNF-alpha and IL-1 were detected in sham-burned animals. Strikingly, IL-1beta levels remained significantly elevated in the lung after injury in animals having 20% and 40% burned skin area. Unexpectedly, both TNF-alpha and IL-1beta production decreased gradually in liver and brain after burn injury. Also, the inflammatory response after a burn injury appeared to be biphasic. The first period corresponded to the early release of IL-6 into the circulation, proportional to the severity of the injury. After a few days, a second period was marked by the extension of the inflammatory processes from the injured area to the rest of the body, particularly to lung, which could be considered as at potential risk of involvement in severely burned patients.
以往的研究表明，热损伤后，人类和动物的全身循环中会早期出现炎性细胞因子。本研究的目的是评估严重烧伤大鼠热损伤后第一周血清、肺、肝和脑中几种细胞因子（IL-6、TNF-α和IL-1β）的时间进程。细胞因子的测定采用酶联免疫吸附测定法（ELISA）。通过比较假烧伤动物和全身表面积20%或40%三度烧伤的动物，研究了与损伤大小相关的炎症过程。血清IL-6水平在假处理动物中无法检测到，在损伤后的最初几个小时达到峰值，且与烧伤面积大小成正比。几天后，IL-6再次升高，但仅在烧伤最严重的大鼠中出现。在肺、肝和脑中，假烧伤动物中检测到低水平但可测量的TNF-α和IL-1基础水平。令人惊讶的是，皮肤烧伤面积为20%和40%的动物损伤后肺中的IL-1β水平仍显著升高。出乎意料的是，烧伤后肝和脑中TNF-α和IL-1β的产生均逐渐减少。此外，烧伤后的炎症反应似乎是双相的。第一阶段对应于IL-6早期释放到循环中，与损伤的严重程度成正比。几天后，第二阶段的特征是炎症过程从损伤部位扩展到身体其他部位，特别是肺，在严重烧伤患者中肺可能被视为有受累的潜在风险。
