Shimizu T, Tomita Y, Son K, Nishinarita S, Sawada S, Horie T
First Department of Internal Medicine, Nihon University School of Medicine, Tokyo, Japan.
Clin Rheumatol. 2000;19(5):352-9. doi: 10.1007/s100670070027.
The aim of the study was, to examine the relationship between serum levels of soluble tumour necrosis factor receptors (sTNF-R) and the gene expression of two types of receptor for TNF (TNF-R), a 55 a receptor (TNF-R1) and a 75 kDa receptor (TNF-R2), bloodin peripheral mononuclear cells (PBMC) from patients with polymyositis and dermatomyositis (PM/DM). Soluble tumour necrosis factor receptor 1 (sTNF-R1) and soluble tumour necrosis factor receptor 2 (sTNF-R2) levels in sera from patients were measured by enzyme-linked immunosorbent assay. Expression of TNF-R1 and TNF-R2 mRNAs in PBMC was analysed by Northern blotting. Serum sTNF-R1 and sTNF-R2 levels were elevated significantly in 25 patients with active-stage PM/DM, compared to those in 18 patients with inactive-stage PM/DM and 32 normal controls. Serum concentrations of sTNF-R1 and sTNF-R2 were correlated with PM/DM disease activity. TNF-R1 gene expression was enhanced in freshly isolated PBMC from patients with active-stage PM/DM. In contrast, TNF-R2 mRNA was expressed constitutively in patients with active-stage PM/DM and in normal controls. The expression of TNF-R1 and TNF-R2 mRNAs in PBMC and elevation of their soluble forms in the sera of patients with active-stage PM/DM suggest increased proteolytic cleavage of cell surface TNF-R from PBMC in patients with active-stage PM/DM, and that sTNF-R may regulate TNF-alpha-mediated muscle fibre damage in PM/DM.
本研究的目的是检测多发性肌炎和皮肌炎（PM/DM）患者外周血单个核细胞（PBMC）中血清可溶性肿瘤坏死因子受体（sTNF-R）水平与两种肿瘤坏死因子（TNF）受体，即55 kDa受体（TNF-R1）和75 kDa受体（TNF-R2）基因表达之间的关系。采用酶联免疫吸附测定法检测患者血清中可溶性肿瘤坏死因子受体1（sTNF-R1）和可溶性肿瘤坏死因子受体2（sTNF-R2）的水平。通过Northern印迹法分析PBMC中TNF-R1和TNF-R2 mRNA的表达。与18例非活动期PM/DM患者和32例正常对照相比，25例活动期PM/DM患者血清sTNF-R1和sTNF-R2水平显著升高。血清sTNF-R1和sTNF-R2浓度与PM/DM疾病活动度相关。活动期PM/DM患者新鲜分离的PBMC中TNF-R1基因表达增强。相反，活动期PM/DM患者和正常对照中TNF-R2 mRNA呈组成性表达。活动期PM/DM患者PBMC中TNF-R1和TNF-R2 mRNA的表达及其血清中可溶性形式的升高表明，活动期PM/DM患者PBMC中细胞表面TNF-R的蛋白水解切割增加，且sTNF-R可能调节PM/DM中TNF-α介导的肌纤维损伤。
