Mohyuddin Aisha, Ayub Qasim, Siddiqi Saima, Carvalho-Silva Denise R, Mazhar Kehkashan, Rehman Sadia, Firasat Sadaf, Dar Ahsana, Tyler-Smith Chris, Mehdi S Qasim
Biomedical and Genetic Engineering Division, Dr. A. Q. Khan Research Laboratories, G.P.O. Box 2891, Islamabad 44000, Pakistan.
Biochim Biophys Acta. 2004 Jan 5;1670(1):81-3. doi: 10.1016/j.bbagen.2003.10.014.
Epstein Barr virus (EBV)-transformed lymphoblastoid cell lines are commonly used to provide an inexhaustible supply of DNA. We examined microsatellite instability in these cell lines in 35 individuals where DNA was available from the original blood samples and from cultured cell lines. Mutations were observed in 0.3% of the analyses, thus providing a quantitative measure of somatic mutation rate.
爱泼斯坦-巴尔病毒（EBV）转化的淋巴母细胞系通常用于提供源源不断的DNA。我们在35名个体的这些细胞系中检测了微卫星不稳定性，这些个体的原始血样和培养细胞系中均有DNA可用。在0.3%的分析中观察到了突变，从而提供了体细胞突变率的定量测量。
