Meldolesi Jacopo, Chieregatti Evelina, Luisa Malosio Maria
Vita-Salute San Raffaele University and Scientific Institute San Raffaele, Department of Neuroscience and Immunology, Centre of Excellence in Physiopathology of Cell Differentiation, via Olgettina 58, 20132 Milan, Italy.
Trends Cell Biol. 2004 Jan;14(1):13-9. doi: 10.1016/j.tcb.2003.11.006.
Dense-core granules (DCGs), cytoplasmic organelles competent for regulated exocytosis, show considerable heterogeneity depending upon the specificity of their expressing cells--primarily neurons and neurosecretory cells. DCGs have been mainly identified by detecting their cargo molecules, often members of the granin family, and using conventional electron microscopy and immunocytochemistry. However, by a critical analysis of the various stages of DCG "life" within neurosecretory cells, we have highlighted several specific molecular and functional properties that are common to all these organelles. We propose that these properties be considered as strict requirements for the identification of DCGs.
致密核心颗粒（DCGs）是能够进行调节性胞吐作用的细胞质细胞器，根据其表达细胞——主要是神经元和神经分泌细胞——的特异性，表现出相当大的异质性。DCGs主要通过检测其货物分子（通常是颗粒蛋白家族的成员）以及使用传统电子显微镜和免疫细胞化学来识别。然而，通过对神经分泌细胞内DCG“生命”各个阶段的批判性分析，我们突出了所有这些细胞器共有的几个特定分子和功能特性。我们建议将这些特性视为鉴定DCGs的严格要求。
