Staples Richard C
Boyce Thompson Institute, Cornell University, Tower Road, Ithaca, NY 14853, USA.
Trends Plant Sci. 2004 Jan;9(1):5-6. doi: 10.1016/j.tplants.2003.10.009.
The late blight fungus (Phytophthora infestans) rots susceptible species of potato plants. None of the major varieties of potato (Solanum tuberosum) grown in the USA is resistant to US-8, the most prevalent genotype of the fungus. Now, Junqi Song, James Bradeen and colleagues have cloned the RB gene from the wild diploid potato species, Solanum bulbocastanum, using a map-based approach in combination with long-range PCR. Transgenic plants containing the gene, normally fully susceptible, displayed broad-spectrum late blight resistance.
晚疫病真菌(致病疫霉)会使易感品种的马铃薯植株腐烂。在美国种植的主要马铃薯品种(茄属马铃薯)中,没有一个对该真菌最普遍的基因型US-8具有抗性。现在,宋俊奇、詹姆斯·布雷登及其同事利用基于图谱的方法结合长片段PCR,从野生二倍体马铃薯物种——块茎茄中克隆出了RB基因。含有该基因的转基因植物通常完全易感,但却表现出了广谱晚疫病抗性。