Williams Patricia A M, Barrio Daniel A, Etcheverry Susana B, Baran Enrique J
Centro de Química Inorgánica, CONICET, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, C. Correo 962, 1900 La Plata, Argentina.
J Inorg Biochem. 2004 Feb;98(2):333-42. doi: 10.1016/j.jinorgbio.2003.10.019.
Oxovanadium (IV) complexes of the alpha-hydroxycarboxylic ligands D-gluconic and D-saccharic acids of stoichiometry Na(2)[VO(gluconate)(2)].H(2)O, K(2)[VO(saccharate)(2)].4H(2)O, Na(4)[VO(gluconate)(2)].2H(2)O and K(5)[VO(saccharate)(2)].4H(2)O were obtained in aqueous solutions; the first two in acid, the other two in alkaline media. They were characterized by infrared and UV-Vis spectroscopies, thermoanalytical (thermogravimetric and differential thermal analysis) data and magnetic susceptibility measurements. The complexes were found to be mononuclear, possessing the VO(2+) moiety, and the thorough analysis of the spectral data allowed the determination of the characteristics of the metal-to-ligand interactions. The biological activities of these complexes on the proliferation, differentiation and glucose consumption were tested on osteoblast-like cells in culture. Comparisons of these effects and those of the oxovanadium (IV) cation and the free ligands were performed. Different behaviors could be observed for the complexes obtained at acidic or alkaline pH-values, as well as for the different cellular types. The free ligands did not show any biological effect.
通过化学计量比为Na(2)[VO(葡萄糖酸盐)(2)].H(2)O、K(2)[VO(糖酸盐)(2)].4H(2)O、Na(4)[VO(葡萄糖酸盐)(2)].2H(2)O和K(5)[VO(糖酸盐)(2)].4H(2)O的α-羟基羧酸配体D-葡萄糖酸和D-糖酸的氧钒(IV)配合物在水溶液中制得；前两种在酸性条件下，另外两种在碱性介质中制得。它们通过红外光谱和紫外可见光谱、热分析（热重分析和差示热分析）数据以及磁化率测量进行表征。发现这些配合物为单核，含有VO(2+)部分，对光谱数据的全面分析有助于确定金属与配体相互作用的特征。在培养的成骨样细胞上测试了这些配合物对增殖、分化和葡萄糖消耗的生物活性。对这些效应与氧钒(IV)阳离子和游离配体的效应进行了比较。对于在酸性或碱性pH值下获得的配合物以及不同的细胞类型，可以观察到不同的行为。游离配体未显示任何生物效应。
