Szelag Adam, Magdalan Jan, Kopacz Maria, Kuźniar Anna, Kowalski Przemysław, Pieśniewska Małgorzata
Department of Pharmacology, Medical University of Wrocław, Mikulicza-Radeckiego 2, PL 50-345 Wrocław, Poland.
Pol J Pharmacol. 2003 Nov-Dec;55(6):1097-103.
Hexavalent chromium compounds exhibit higher toxicity than its trivalent compounds since chromium ions in the +6 oxidation state easily cross biological membranes. It has recently been proposed that substances reducing chromium ions from the +6 to the less toxic +3 oxidation state can be beneficial in management of acute chromium poisoning. In vitro studies also demonstrated quercetin-5 '-sulfonic acid sodium salt (NaQSA) to reduce chromium ions from the +6 to the +3 oxidation state. The aim of the study was to determine efficacy of NaQSA in treatment of acute poisoning with a hexavalent chromium compound. The experiment was carried out on male and female Wistar rats which were divided into 4 experimental (A,B,C,D) and control (K) groups. All animals received intragastrically a single CrO3 dose equal to its LD50. Thirty minutes after administration of CrO3, NaQSA was administered intragastrically at a dose of 50 mg/kg (group A) and 100 mg/kg (group B). In groups C and D, NaQSA was administered ip 2 h after administration of CrO3 and then twice a day for 4 days at doses of 50 mg/kg (group C) and 100 mg/kg (group D). Only intragastric administration of NaQSA at a dose of 100 mg/kg decreased mortality in acute poisoning with CrO3. In groups B and D, aminotransferase activity was statistically significantly dropping from day 7 of the experiment in comparison with the group K, which indicates lesser damage to the liver in animals treated with NaQSA. Bilirubin concentrations in groups B and D were also much lower than in the group K, but the difference between average bilirubin levels in these groups and the K was not statistically significant. The results of the study suggest the usefulness of NaQSA in the treatment of poisoning with hexavalent chromium compounds.
六价铬化合物比其三价化合物具有更高的毒性，因为处于+6氧化态的铬离子很容易穿过生物膜。最近有人提出，将铬离子从+6氧化态还原为毒性较低的+3氧化态的物质可能有助于治疗急性铬中毒。体外研究还表明，槲皮素-5'-磺酸钠盐（NaQSA）能将铬离子从+6氧化态还原为+3氧化态。本研究的目的是确定NaQSA治疗六价铬化合物急性中毒的疗效。实验选用雄性和雌性Wistar大鼠，分为4个实验组（A、B、C、D）和对照组（K）。所有动物均经胃内给予单一剂量的CrO3，其剂量等于半数致死量（LD50）。给予CrO3 30分钟后，以50mg/kg（A组）和100mg/kg（B组）的剂量经胃内给予NaQSA。在C组和D组中，在给予CrO3 2小时后经腹腔注射给予NaQSA，然后连续4天每天两次，剂量分别为50mg/kg（C组）和100mg/kg（D组）。只有经胃内给予100mg/kg剂量的NaQSA能降低CrO3急性中毒的死亡率。在B组和D组中，与K组相比，从实验第7天起转氨酶活性有统计学意义的下降，这表明用NaQSA治疗的动物肝脏损伤较小。B组和D组的胆红素浓度也远低于K组，但这些组与K组的平均胆红素水平差异无统计学意义。研究结果表明NaQSA在治疗六价铬化合物中毒方面是有用的。
