Qian Xiao-wei, Luo Wei-hua, Zheng Ou-xiang
School of Life and Environmental Science, Wenzhou University, Wenzhou 325003, China.
J Zhejiang Univ Sci B. 2006 Mar;7(3):221-7. doi: 10.1631/jzus.2006.B0221.
The mutagenic effects of microwave and chromium trioxide (CrO(3)) on Vicia faba root tip were studied. Micronucleus assay and chromosomal aberration assay were used to determine the mitotic index, the micronucleus frequency and chromosomal aberration frequency of Vicia faba root tip cells induced by microwave and CrO(3). The results showed that the micronucleus frequency decreased, and that the mitotic index and chromosomal aberration frequency showed linear dose responses to CrO(3), in treatment of microwave for 5 s. In microwave of 25 s, the mitotic index decreased, the micronucleus frequency and chromosomal aberration frequency increased with increase of CrO(3) concentration. We concluded that microwave and CrO(3) had antagonistic effect on the mitotic index of Vicia faba root tip cells, but had synergetic effect on micronucleus frequency and chromosomal aberration frequency of Vicia faba root tip cells.
研究了微波和三氧化铬(CrO₃)对蚕豆根尖的诱变效应。采用微核试验和染色体畸变试验来测定微波和CrO₃诱导的蚕豆根尖细胞的有丝分裂指数、微核频率和染色体畸变频率。结果表明,在微波处理5 s时,微核频率降低,有丝分裂指数和染色体畸变频率对CrO₃呈线性剂量反应。在微波处理25 s时,随着CrO₃浓度的增加,有丝分裂指数降低,微核频率和染色体畸变频率增加。我们得出结论,微波和CrO₃对蚕豆根尖细胞的有丝分裂指数具有拮抗作用,但对蚕豆根尖细胞的微核频率和染色体畸变频率具有协同作用。