Department of Pharmacology, Wroclaw Medical University, Poland.
Adv Clin Exp Med. 2012 Jul-Aug;21(4):423-31.
Quercetin-5'-sulfonic acid sodium salt (NaQSA) exerts good aqueous solubility, strong antioxidant activity and low toxicity.
The aims of this study were to investigate the effect of NaQSA on superoxide dismutase (SOD) activity and ADMA/DDAH pathway during extracorporeal liver perfusion (ELP).
The study was carried out on male Wistar rats. Isolated livers were perfused with Krebs-Henseleit bicarbonate buffer (KHB) + 1 microM ADMA (group C), or with KHB + 1 microM ADMA and either 10 microM NaQSA (Q10) or 50 microM NaQSA (Q50). In group 0 (sham) livers were perfused with KHB alone. Levels of ADMA, alanine (ALT) and aspartate (AST) aminotransferases activities were measured during perfusion. After 90 min. of perfusion superoxide dismutase (SOD) and dimethylarginine dimethylaminohydrolase (DDAH) activities were estimated in liver homogenates.
DDAH activity in Q10 group was significantly higher as compared to control and Q50 groups. No significant differences were observed between Q50 and control group. The decrease in ADMA concentration during perfusion was observed in all groups, but the most pronounced in the group Q10 and the least in group Q50. During perfusion AST activities were the lowest in Q50 group. No significant difference in SOD activity in groups perfused with NaQSA as compared to control group was noted.
The impact of NaQSA on ADMA/DDAH system depends on its concentration. In lower concentration NaQSA exerted some beneficial properties which vanished in higher concentration. No increase in SOD activity during perfusion with NaQSA was observed.
槲皮素-5'-磺酸纳（NaQSA）具有良好的水溶性、较强的抗氧化活性和较低的毒性。
本研究旨在探讨 NaQSA 对体外肝灌注（ELP）中超氧化物歧化酶（SOD）活性和 ADMA/DDAH 途径的影响。
本研究在雄性 Wistar 大鼠中进行。分离的肝脏用 Krebs-Henseleit 碳酸氢盐缓冲液（KHB）+1μM ADMA（C 组）进行灌注，或用 KHB+1μM ADMA 加 10μM NaQSA（Q10）或 50μM NaQSA（Q50）进行灌注。在 0 组（假手术）中，肝脏仅用 KHB 灌注。在灌注过程中测量 ADMA、丙氨酸（ALT）和天冬氨酸（AST）氨基转移酶的活性。灌注 90 分钟后，在肝匀浆中测定超氧化物歧化酶（SOD）和二甲基精氨酸二甲氨基水解酶（DDAH）的活性。
与对照组和 Q50 组相比，Q10 组的 DDAH 活性显著升高。Q50 组与对照组之间无显著差异。在所有组中均观察到 ADMA 浓度在灌注过程中的降低，但在 Q10 组中降低最明显，在 Q50 组中降低最不明显。在 Q50 组中，AST 活性在灌注过程中最低。与对照组相比，用 NaQSA 灌注的各组中 SOD 活性无显著差异。
NaQSA 对 ADMA/DDAH 系统的影响取决于其浓度。在较低浓度下，NaQSA 表现出一些有益的特性，而在较高浓度下这些特性消失。在 NaQSA 灌注过程中未观察到 SOD 活性的增加。
