Yasuda Michiko, Nishioka Masanori, Nakashita Hideo, Yamaguchi Isamu, Yoshida Shigeo
Laboratory for Growth Regulation, Plant Science Center, RIKEN, Yokohama, Saitama, Japan.
Biosci Biotechnol Biochem. 2003 Dec;67(12):2614-20. doi: 10.1271/bbb.67.2614.
Systemic acquired resistance (SAR) is a potent innate immunity system in plants that is induced through asalicylic acid (SA)-mediated pathway. Here, we characterized 3-chloro-1-methyl-1H-pyrazole-5-carboxylic acid (CMPA) as an effective SAR inducer in tobacco. Soil drench application of CMPA induced PR gene expression and a broad range of disease resistance without antibacterial activity in tobacco. Both analysis of CMPA's effects on NahG transgenic tobacco plants and SA measurement in wild-type plants indicated that CMPA-induced resistance enhancement does not require SA. Therefore, it is suggested that CMPA induces SAR by triggering the signaling at the same level as or downstream of SA accumulation as do both benzo(1,2,3)thiadiazole-7-carbothioic acid S-methyl ester and N-cyanomethyl-2-chloroisonicotinamide.
系统获得性抗性（SAR）是植物中一种强大的固有免疫系统，通过水杨酸（SA）介导的途径诱导产生。在此，我们将3-氯-1-甲基-1H-吡唑-5-羧酸（CMPA）鉴定为烟草中一种有效的SAR诱导剂。土壤浇灌CMPA可诱导烟草中病程相关（PR）基因表达和广泛的抗病性，且无抗菌活性。对CMPA对NahG转基因烟草植株的影响分析以及野生型植株中SA的测量均表明，CMPA诱导的抗性增强不需要SA。因此，有人提出，与苯并[1,2,3]噻二唑-7-硫代羧酸S-甲酯和N-氰基甲基-2-氯异烟酰胺一样，CMPA通过在与SA积累相同水平或其下游触发信号传导来诱导SAR。
