Yasuda Michiko, Nakashita Hideo, Hasegawa Satoru, Nishioka Masanori, Arai Yuko, Uramoto Masakazu, Yamaguchi Isamu, Yoshida Shigeo
Plant Functions Laboratory, RIKEN Institute, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
Biosci Biotechnol Biochem. 2003 Feb;67(2):322-8. doi: 10.1271/bbb.67.322.
Systemic acquired resistance (SAR) is a potent innate immunity system in plants that is induced through the salicylic acid-mediated pathway. N-cyanomethyl-2-chloroisonicotinamide (NCI) is able to induce a broad range of disease resistance in tobacco and rice and induces SAR marker gene expression without SA accumulation in tobacco. To clarify the detailed mode of action of NCI, we analyzed its ability to induce defense gene expression and resistance in Arabidopsis mutants that are defective in various defense signaling pathways. Wild-type Arabidopsis treated with NCI exhibited increased expression of several pathogenesis-related genes and enhanced resistance to the bacterial pathogen, Pseudomonas syringae pv. tomato DC3000. NCI induced disease resistance and PR gene expression in NahG transgenic plants, but not in the npr1 mutant. NCI could induce PR gene expression in the etr1-1, ein2-1 and jar1-1 mutants. Thus, NCI activates SAR, independently from ethylene and jasmonic acid, by stimulating the site between SA and NPR1.
系统获得性抗性（SAR）是植物中一种强大的先天免疫系统，通过水杨酸介导的途径诱导产生。N-氰甲基-2-氯异烟酰胺（NCI）能够在烟草和水稻中诱导广泛的抗病性，并且在烟草中诱导SAR标记基因表达而不积累水杨酸。为了阐明NCI的详细作用模式，我们分析了它在各种防御信号通路有缺陷的拟南芥突变体中诱导防御基因表达和抗性的能力。用NCI处理的野生型拟南芥表现出几种病程相关基因的表达增加，并增强了对细菌病原体丁香假单胞菌番茄致病变种DC3000的抗性。NCI在NahG转基因植物中诱导抗病性和病程相关基因表达，但在npr1突变体中则不能。NCI能够在etr1-1、ein2-1和jar1-1突变体中诱导病程相关基因表达。因此，NCI通过刺激水杨酸和NPR1之间的位点，独立于乙烯和茉莉酸激活系统获得性抗性。
