Zhang Jiyou, Xie Jianping, Liu Jiaqin, Tian Jianniao, Chen Xingguo, Hu Zhide
Department of Chemistry, Lanzhou University, Lanzhou, China.
Electrophoresis. 2004 Jan;25(1):74-9. doi: 10.1002/elps.200305643.
A selective and sensitive microemulsion electrokinetic chromatography with laser-induced fluorescence detection method was developed for the quantification of ephedrine (E) and pseudoephedrine (PE) derivatized with 4-chloro-7-nitrobenzo-2-oxa-1, 3-diazol. By a series of optimization, a running buffer composed of 20 mM borate + microemulsion (23.3 mM Sodium dodecyl sulfate/180.85 mM 1-butanol/16.4 mM n-heptane) +8% acetonitrile was applied for the separation of the derivatives. A linear relationship for E and PE was obtained in the range of 0.058-11.58 microg.mL(-1) (correlation coefficient: 0.9993 for E, 0.9995 for PE), and the detection limits for E and PE were 5.3 and 3.9 ng.mL(-1). The method was applied to the analysis of the two alkaloids in Chinese traditional herbal preparations with recoveries in the range of 96.9-105.4%.
建立了一种选择性灵敏的微乳液电动色谱-激光诱导荧光检测方法，用于定量分析用4-氯-7-硝基苯并-2-恶唑-1,3-二唑衍生化的麻黄碱（E）和伪麻黄碱（PE）。通过一系列优化，采用由20 mM硼酸盐+微乳液（23.3 mM十二烷基硫酸钠/180.85 mM正丁醇/16.4 mM正庚烷）+8%乙腈组成的运行缓冲液对衍生物进行分离。E和PE在0.058-11.58 μg·mL⁻¹范围内呈线性关系（相关系数：E为0.9993，PE为0.9995），E和PE的检测限分别为5.3和3.9 ng·mL⁻¹。该方法应用于中药制剂中两种生物碱的分析，回收率在96.9-105.4%范围内。
