Chetty Anne, Andersson Sture, Lassus Patrik, Nielsen Heber C
Division of Pulmonology, Department of Pediatrics, Floating Hospital for Children, Tufts-New England Medical Center, Boston, Massachusetts, USA.
Pediatr Pulmonol. 2004 Feb;37(2):128-36. doi: 10.1002/ppul.10415.
We hypothesize that IGF-1 and IGF-1R proteins are upregulated in lung epithelia and fibroblasts in RDS compared to normal development, and are further upregulated in BPD. We used immunohistochemistry to evaluate IGF-1 and IGF-R expression in lungs from autopsies of human stillbirths and RDS and BPD patients. IGF-1 and IGF-R immunostaining were present in fetal, RDS, and BPD lungs. In RDS, IGF-1 was present in alveolar epithelium and prominent in columnar and cuboidal airway epithelia. In BPD lungs, immunostaining was intensely increased in both airway and alveolar epithelia and in mesenchyme. The immunostaining index in bronchial epithelial cells and peribronchial myofibroblasts was significantly higher in BPD compared to RDS. IGF-1R expression was minimal in fetal lung and found mainly in mesenchyme. IGF-1R was increased in mesenchyme in RDS. In BPD it was especially increased in peribronchial and perialveolar mesenchyme. Immunostaining index for IGF-1R in epithelial cells and peribronchial myofibroblasts was increased in BPD compared to RDS. IGF-1 and IGF-R expression is low during fetal development, but is acutely upregulated in RDS, and persists with further upregulation in BPD.
我们推测，与正常发育相比，呼吸窘迫综合征（RDS）患者肺上皮细胞和成纤维细胞中的胰岛素样生长因子-1（IGF-1）和胰岛素样生长因子-1受体（IGF-1R）蛋白上调，并且在支气管肺发育不良（BPD）中进一步上调。我们使用免疫组织化学方法评估了人类死产儿、RDS和BPD患者尸检肺组织中IGF-1和IGF-R的表达。IGF-1和IGF-R免疫染色在胎儿、RDS和BPD肺组织中均有出现。在RDS中，IGF-1存在于肺泡上皮，在柱状和立方状气道上皮中尤为明显。在BPD肺组织中，气道和肺泡上皮以及间充质中的免疫染色均显著增加。与RDS相比，BPD患者支气管上皮细胞和支气管周围肌成纤维细胞中的免疫染色指数显著更高。IGF-1R在胎儿肺中的表达极少，主要存在于间充质中。在RDS中，IGF-1R在间充质中增加。在BPD中，它在支气管周围和肺泡周围间充质中尤其增加。与RDS相比，BPD患者上皮细胞和支气管周围肌成纤维细胞中IGF-1R的免疫染色指数增加。IGF-1和IGF-R的表达在胎儿发育期间较低，但在RDS中急剧上调，并在BPD中持续进一步上调。
