Pedersen K, Dietz H H, Jørgensen J C, Christensen T K, Bregnballe T, Andersen T H
Danish Veterinary Institute, Department of Poultry, Fish, and Fur Animals, Hangøvej 2, DK-8200 Aarhus N, Denmark.
J Wildl Dis. 2003 Oct;39(4):808-16. doi: 10.7589/0090-3558-39.4.808.
An outbreak of avian cholera was observed among wild birds in a few localities in Denmark in 2001. The highest mortalities were among breeding eiders (Somateria mollissima) and gulls (Larus spp.). Pulsed-field gel electrophoresis (PFGE) was conducted using ApaI and SmaI as restriction enzymes and restriction enzyme analysis (REA) using HpaII. The Pasteurella multocida subsp. multocida strain isolated from birds in this outbreak was indistinguishable from a strain that caused outbreaks in 1996 and 2003. Most isolates from domestic poultry had other PFGE patterns but some were indistinguishable from the outbreak strain. Among 68 isolates from wild birds, only one PFGE and one REA pattern were demonstrated, whereas among 23 isolates from domestic poultry, 14 different SmaI, 12 different ApaI, and 10 different HpaII patterns were found. The results suggest that a P. multocida strain has survived during several years among wild birds in Denmark.
2001年在丹麦的一些地区观察到野生鸟类中爆发了禽霍乱。死亡率最高的是繁殖期的绒鸭（Somateria mollissima）和海鸥（Larus spp.）。使用ApaI和SmaI作为限制酶进行脉冲场凝胶电泳（PFGE），并使用HpaII进行限制酶分析（REA）。从此次疫情中鸟类分离出的多杀巴斯德氏菌多杀亚种菌株与1996年和2003年引起疫情的菌株无法区分。来自家禽的大多数分离株有其他PFGE模式，但有些与疫情菌株无法区分。在68株来自野生鸟类的分离株中，仅显示出一种PFGE和一种REA模式，而在23株来自家禽的分离株中，发现了14种不同的SmaI、12种不同的ApaI和10种不同的HpaII模式。结果表明，一种多杀巴斯德氏菌菌株在丹麦的野生鸟类中存活了数年。
