Rudolph Karen M, Hunter David L, Foreyt William J, Cassirer E Frances, Rimler Richard B, Ward Alton C S
Idaho Department of Fish and Game, Wildlife Health Laboratory, 16569 S10th Avenue, Caldwell, Idaho 83607, USA.
J Wildl Dis. 2003 Oct;39(4):897-903. doi: 10.7589/0090-3558-39.4.897.
Pasteurella spp. were isolated from feral goats and free-ranging bighorn sheep (Ovis canadensis canadensis) in the Hells Canyon National Recreation Area bordering Idaho, Oregon, and Washington (USA). Biovariant 1 Pasteurella haemolytica organisms were isolated from one goat and one of two bighorn sheep found in close association. Both isolates produced leukotoxin and had identical electrophoretic patterns of DNA fragments following cutting with restriction endonuclease HaeIII. Similarly Pasteurella multocida multocida a isolates cultured from the goat and one of the bighorn sheep had D type capsules, serotype 4 somatic antigens, produced dermonecrotoxin and had identical HaeIII electrophoretic profiles. A biovariant U(beta) P.haemolytica strain isolated from two other feral goats, not known to have been closely associated with bighorn sheep, did not produce leukotoxin but had biochemical utilization and HaeIII electrophoretic profiles identical to those of isolates from bighorn sheep. It was concluded that identical Pasteurella strains were shared by the goats and bighorn sheep. Although the direction of transmission could not be established, evidence suggests transmission of strains from goats to bighorn sheep. Goats may serve as a reservoir of Pasteurella strains that may be virulent in bighorn sheep; therefore, goats in bighorn sheep habitat should be managed to prevent contact with bighorn sheep. Bighorn sheep which have nose-to-nose contact with goats should be removed from the habitat.
在美国爱达荷州、俄勒冈州和华盛顿州交界处的地狱峡谷国家游乐区，从野生山羊和自由放养的大角羊（加拿大盘羊指名亚种）中分离出了巴氏杆菌属细菌。从一只山羊和与之密切接触的两只大角羊中的一只身上分离出了生物变种1溶血巴氏杆菌。两种分离株均产生白细胞毒素，在用限制性内切酶HaeIII切割后，其DNA片段具有相同的电泳图谱。同样，从山羊和一只大角羊身上培养出的多杀性巴氏杆菌多杀亚种a分离株具有D型荚膜、4型菌体抗原，产生皮肤坏死毒素，并且具有相同的HaeIII电泳图谱。从另外两只野生山羊中分离出的一株生物变种U(β)溶血巴氏杆菌，已知其与大角羊没有密切接触，该分离株不产生白细胞毒素，但在生化利用和HaeIII电泳图谱方面与从大角羊中分离出的菌株相同。得出的结论是，山羊和大角羊共享相同的巴氏杆菌菌株。尽管无法确定传播方向，但有证据表明菌株是从山羊传播到大角羊的。山羊可能是巴氏杆菌菌株的储存宿主，这些菌株可能对大角羊具有致病性；因此，应该对大角羊栖息地的山羊进行管理，以防止它们与大角羊接触。与山羊有鼻对鼻接触的大角羊应该从栖息地中移除。
