Tomassini Letizia, Gonzales Ben, Weiser Glen C, Sischo William
Washington State University, PO Box 646610, Pullman, Washington 99164-6610, USA.
J Wildl Dis. 2009 Oct;45(4):930-40. doi: 10.7589/0090-3558-45.4.930.
The prevalence and phenotypic variability of Pasteurella and Mannheimia isolates from Sierra Nevada bighorn sheep (Ovis canadensis sierrae), White Mountain bighorn sheep (Ovis canadensis nelsoni), and domestic sheep (Ovis aries) from California, USA, were compared. The White Mountain bighorn sheep population had a recent history of pneumonia-associated mortality, whereas the Sierra Nevada bighorn sheep population had no recent history of pneumonia-associated mortality. The domestic sheep flocks were pastured in areas geographically near both populations but were not known to have direct contact with either bighorn sheep population. Oropharyngeal swab samples were collected from healthy domestic and bighorn sheep and cultured to characterize bacterial species, hemolysis, biogroups, and biovariants. Pasteurella trehalosi and Mannheimia haemolytica were detected in all of the study populations, but the relative proportion of each bacterial species differed among sheep populations. Pasteurella trehalosi was more common than M. haemolytica in the bighorn sheep populations, whereas the opposite was true in domestic sheep. Mannheimia haemolytica was separated into 11 biogroups, and P. trehalosi was characterized into two biogroups. Biogroup distributions for M. haemolytica and P. trehalosi differed among the three populations; however, no difference was detected for the distribution of P. trehalosi biogroups between the Sierra Nevada bighorn sheep and domestic sheep. The prevalence odds ratios (pOR) for the distribution of M. haemolytica biogroups suggested little difference between White Mountain bighorn sheep and domestic sheep compared with Sierra Nevada bighorn sheep and domestic sheep, although these comparisons had relatively large confidence intervals for the point estimates. Hemolytic activity of the isolates was not different among the sheep populations for M. haemolytica but was different for P. trehalosi. No clear evidence of association was found in the Pasteurellaceae distribution between the White Mountains bighorn sheep and domestic sheep.
对来自美国加利福尼亚州内华达山脉大角羊（加拿大盘羊内华达亚种，Ovis canadensis sierrae）、白山大角羊（加拿大盘羊尼尔森亚种，Ovis canadensis nelsoni）以及家养绵羊（Ovis aries）的巴斯德氏菌属和曼氏杆菌属分离株的流行情况及表型变异性进行了比较。白山大角羊种群近期有与肺炎相关的死亡病史，而内华达山脉大角羊种群近期无与肺炎相关的死亡病史。家养绵羊群在地理上靠近这两个种群的区域放牧，但未知与任何一个大角羊种群有直接接触。从健康的家养绵羊和大角羊采集口咽拭子样本并进行培养，以鉴定细菌种类、溶血情况、生物群和生物变种。在所有研究种群中均检测到了海藻糖巴斯德氏菌（Pasteurella trehalosi）和溶血曼氏杆菌（Mannheimia haemolytica），但各细菌种类的相对比例在不同绵羊种群中有所差异。在大角羊种群中，海藻糖巴斯德氏菌比溶血曼氏杆菌更常见，而在家养绵羊中情况则相反。溶血曼氏杆菌被分为11个生物群，海藻糖巴斯德氏菌被分为两个生物群。溶血曼氏杆菌和海藻糖巴斯德氏菌的生物群分布在三个种群之间存在差异；然而，在内华达山脉大角羊和家养绵羊之间，未检测到海藻糖巴斯德氏菌生物群分布的差异。溶血曼氏杆菌生物群分布的患病率比值比（pOR）表明，与内华达山脉大角羊和家养绵羊相比，白山大角羊和家养绵羊之间差异不大，尽管这些比较的点估计置信区间相对较大。对于溶血曼氏杆菌，各绵羊种群分离株的溶血活性无差异，但对于海藻糖巴斯德氏菌则有差异。在白山大角羊和家养绵羊之间，巴斯德氏菌科的分布未发现明显的关联证据。
