Hartig Jörg S, Grüne Imke, Najafi-Shoushtari S Hani, Famulok Michael
Kekulé Institut für Organische Chemie und Biochemie, Universität Bonn, Gerhard-Domagk-Strasse 1, 53121 Bonn, Germany.
J Am Chem Soc. 2004 Jan 28;126(3):722-3. doi: 10.1021/ja038822u.
The rational and straightforward design of hairpin ribozymes that can be sequence-specifically induced by external oligonucleotides is described. Due to intrinsic signal amplification, their sensitivity is at least an order of magnitude increased compared to standard molecular beacons. We applied this system to the detection of microRNAs, a recently discovered class of small endogenous RNA molecules that are involved in gene regulation. We show that the cognate microRNA can reliably and sensitively be detected at low concentrations in a mix of other microRNA sequences. These probes may be useful in applications that require direct detection of minute amounts of small DNAs or RNAs.
本文描述了一种发夹状核酶的合理且直接的设计，该酶可由外部寡核苷酸进行序列特异性诱导。由于其固有的信号放大作用，与标准分子信标相比，它们的灵敏度至少提高了一个数量级。我们将该系统应用于微小RNA的检测，微小RNA是最近发现的一类参与基因调控的内源性小RNA分子。我们表明，在其他微小RNA序列的混合物中，低浓度的同源微小RNA能够被可靠且灵敏地检测到。这些探针可能在需要直接检测微量小DNA或RNA的应用中有用。
