Wang C R, Chuang C Y, Lin K T, Chen M Y, Lee G L, Chen C Y
Department of Internal Medicine, College of Medicine, National Taiwan University Hospital, Taipei, R.O.C.
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1992 May;25(2):69-77.
The immunochemical characteristics of house dust extract used in hyposensitization were studied by SDS-polyacrylamide gel, isoelectrofocusing, counterimmunoelectrophoresis and Western blot. The gel electrophoregram and isoelectrofocusing plate demonstrated a complex mixture of acid proteins with molecular weights ranging from 56 to 12 KD and isoelectric point distributing from 4.6 to 5.6. The counterimmunoelectrophoresis revealed as many as five precipitating bands in sera from 60% asthmatic patients after hyposensitization. The Western blot showed heterogeneous IgE reaction bands varied from 45 to 22 KD, including a strong 27 KD reaction band found in 40% of the asthmatic patients not receiving hyposensitization. The efficacy of house dust extract hyposensitization was evaluated in 102 asthmatic patients, followed up at the Allergic Clinic since 1980. There were 42 malea and 60 females with a mean age of 30.8 (16 to 60), and the total follow-up patient-year was 512.6 with a mean of 5.0 year per patient (1 to 11). Complete remission of asthmatic symptoms and medication was found in 5% patients, and 12% patients had little or no improvement. The majority of patients (83%) had improvement in asthmatic symptoms and medication dosage. The average serum IgE levels and total eosinophil count dropped significantly after hyposensitization (p < 0.001). In conclusion, the immunochemical characteristics of house dust extract were analyzed and mite allergens, especially Group I antigens, may be the important constituents. Good therapeutic effect, both clinically and with laboratory evaluation, was obtained with more than 80% of the asthmatic patients achieving improvement under house dust hyposensitization.
采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、等电聚焦电泳、对流免疫电泳和蛋白质印迹法研究了用于脱敏治疗的屋尘提取物的免疫化学特性。凝胶电泳图和等电聚焦板显示，酸性蛋白混合物复杂，分子量范围为56至12千道尔顿，等电点分布在4.6至5.6之间。对流免疫电泳显示，60%的哮喘患者在脱敏治疗后血清中有多达五条沉淀带。蛋白质印迹显示，异质性IgE反应带的分子量在45至22千道尔顿之间，其中40%未接受脱敏治疗的哮喘患者有一条强27千道尔顿的反应带。对1980年以来在过敏诊所随访的102例哮喘患者进行了屋尘提取物脱敏治疗的疗效评估。其中男性42例，女性60例，平均年龄30.8岁（16至60岁），总随访患者年数为512.6，平均每位患者5.0年（1至11年）。5%的患者哮喘症状和药物治疗完全缓解，12%的患者改善很少或没有改善。大多数患者（83%）的哮喘症状和药物剂量有所改善。脱敏治疗后，患者血清IgE平均水平和嗜酸性粒细胞总数显著下降（p<0.001）。总之，分析了屋尘提取物的免疫化学特性，螨过敏原尤其是Ⅰ组抗原可能是重要成分。屋尘脱敏治疗对80%以上的哮喘患者有良好的临床和实验室评估治疗效果。
