Lu J R, Wu H C
Department of Microbiology, Chung Shan Medical and Dental College, Taichung, Taiwan, R.O.C.
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1992 May;25(2):91-100.
The purposes of this study were to give the characteristics of viridans streptococci isolated from dental plaque, and the relationships with dental caries. We stained 69 patients' teeth on supragingival plaques (Plaque Index = 1) with the Pfiffer's reagent or FD&C RED#28 dye, then isolated 80 strains of oral streptococci from 69 patients. Cultures were grown in TSB, MS, and MSB media. A total of 80 fresh-isolated plaque streptococci, including strains of S. salivarius, S. sanguis, S. mitis, and S. mutans were identified on the basis of morphologic, dark field, SEM, and biochemical characteristics. All of the oral plaque streptococci were to be found in significant percentages in dental plaque. Patients who had caries harboured S. mutans more often (37/40) than patients without caries (7/29). S. mutans comprised the greatest percentage (37/69) of microorganisms encountered in caries' plaque, and there was an association between high counts of S. mutans and the presence of dental caries. Cultural and biochemical methods were used for the isolation and identification of the different species of S. mutans. The result indicated that biotype I were the most predominant species (50%) in dental plaque. Thus, it is possible to isolate, screen and identify unknown strains through the morphologic, darkfield, SEM, and biochemical characteristics of viridans streptococci isolated from human dental plaque. By means of the present methods for identification, we hold considerable promises for contributing to the prevention and diagnosis of dental diseases.
本研究的目的是给出从牙菌斑中分离出的草绿色链球菌的特征，以及它们与龋齿的关系。我们用普法伊费尔试剂或FD&C RED#28染料对69例患者的龈上菌斑（菌斑指数=1）进行染色，然后从69例患者中分离出80株口腔链球菌。培养物在TSB、MS和MSB培养基中生长。根据形态学、暗视野、扫描电子显微镜和生化特征，共鉴定出80株新鲜分离的菌斑链球菌，包括唾液链球菌、血链球菌、轻链球菌和变形链球菌菌株。所有口腔菌斑链球菌在牙菌斑中的占比都很高。有龋齿的患者比无龋齿的患者更常携带变形链球菌（37/40比7/29）。变形链球菌在龋斑中占微生物的比例最大（37/69），变形链球菌数量高与龋齿的存在之间存在关联。采用培养和生化方法分离和鉴定不同种类的变形链球菌。结果表明，I型生物型是牙菌斑中最主要的种类（50%）。因此，通过从人类牙菌斑中分离出的草绿色链球菌的形态学、暗视野、扫描电子显微镜和生化特征，可以分离、筛选和鉴定未知菌株。通过目前的鉴定方法，我们有望为口腔疾病的预防和诊断做出重要贡献。
