Swenson Dana L, Warfield Kelly L, Kuehl Kathleen, Larsen Thomas, Hevey Michael C, Schmaljohn Alan, Bavari Sina, Aman M Javad
U.S. Army Medical Research Institute of Infectious Diseases, 1425 Porter Street, Frederick, MD 21702, USA.
FEMS Immunol Med Microbiol. 2004 Jan 15;40(1):27-31. doi: 10.1016/S0928-8244(03)00273-6.
Marburg virus (MARV), the causative agent of a severe hemorrhagic fever, has a characteristic filamentous morphology. Here we report that co-expression of MARV glycoprotein and matrix protein (VP40) in mammalian cells leads to spontaneous budding of filamentous particles strikingly similar to wild-type MARV. In addition, these particles elicit an immune response in BALB/c mice. The generation of non-replicating Marburg virus-like particles (VLPs) should significantly facilitate the research on molecular mechanisms of MARV assembly and release. Furthermore, VLPs may be an excellent vaccine candidate against Marburg infection.
马尔堡病毒（MARV）是一种严重出血热的病原体，具有特征性的丝状形态。在此我们报告，在哺乳动物细胞中共同表达马尔堡病毒糖蛋白和基质蛋白（VP40）会导致丝状颗粒的自发芽生，这些颗粒与野生型马尔堡病毒惊人地相似。此外，这些颗粒在BALB/c小鼠中引发免疫反应。非复制性马尔堡病毒样颗粒（VLPs）的产生应能显著促进对马尔堡病毒组装和释放分子机制的研究。此外，VLPs可能是预防马尔堡病毒感染的优秀疫苗候选物。
