Karginov Vladimir A, Robinson Tanisha M, Riemenschneider Jenny, Golding Basil, Kennedy Michael, Shiloach Joseph, Alibek Ken
Advanced Biosystems, Inc., 10900 University Blvd., Manassas, VA 20110, USA.
FEMS Immunol Med Microbiol. 2004 Jan 15;40(1):71-4. doi: 10.1016/S0928-8244(03)00302-X.
Currently there is no effective treatment for inhalational anthrax beyond administration of antibiotics shortly after exposure. There is need for new, safe and effective treatments to supplement traditional antibiotic therapy. Our study was based on the premise that simultaneous inhibition of lethal toxin action with antibodies and blocking of bacterial growth by antibiotics will be beneficial for the treatment of anthrax. In this study, we tested the effects of a combination treatment using purified rabbit or sheep anti-protective antigen (PA) antibodies and the antibiotic ciprofloxacin in a rodent anthrax model. In mice infected with a dose of Bacillus anthracis Sterne strain corresponding to 10 LD(50), antibiotic treatment with ciprofloxacin alone only cured 50% of infected animals. Administration of anti-PA IgG in combination with ciprofloxacin produced 90-100% survival. These data indicate that a combination of antibiotic/immunoglobulin therapy is more effective than antibiotic treatment alone in a rodent anthrax model.
目前，除了在接触后不久使用抗生素外，尚无针对吸入性炭疽的有效治疗方法。需要新的、安全有效的治疗方法来补充传统的抗生素疗法。我们的研究基于这样一个前提，即同时用抗体抑制致死毒素的作用并通过抗生素阻断细菌生长将有利于炭疽的治疗。在本研究中，我们在啮齿动物炭疽模型中测试了使用纯化的兔或羊抗保护性抗原（PA）抗体与抗生素环丙沙星联合治疗的效果。在用相当于10倍半数致死量（LD50）的炭疽芽孢杆菌斯特恩菌株感染的小鼠中，单独使用环丙沙星进行抗生素治疗仅治愈了50%的感染动物。抗PA IgG与环丙沙星联合给药的生存率为90%-100%。这些数据表明，在啮齿动物炭疽模型中，抗生素/免疫球蛋白联合治疗比单独使用抗生素治疗更有效。
