Izevbigie Ernest B, Bryant Joseph L, Walker Alice
The Molecular Genetics and Molecular & Cellular Signaling Laboratory, Department of Biology, and National Institutes of Health Center for Environmental Health, Jackson State University, Mississippi 39217, USA.
Exp Biol Med (Maywood). 2004 Feb;229(2):163-9. doi: 10.1177/153537020422900205.
Water-soluble extracts of edible Vernonia amygdalina leaves were recently reported as potent inhibitors of cultured MCF-7 cells. The mechanism by which V. amygdalina inhibits MCF-7 cell growth has not been previously studied. The objective of this study was to evaluate the effects of V. amygdalina on the activities, DNA synthesis, and subsequent cell growth of extracellular signal-regulated protein kinases 1 and 2 (ERKs 1/2;). Treatment of cells with various concentrations (3-100 mg/ml) of water-soluble V. amygdalina extract potently inhibited ERK activities, DNA synthesis (P < 0.005), and cell growth (P < 0.01) in a concentration-dependent fashion, both in the absence and presence of serum. The growth rate of cells pretreated with 10 mg/ml V. amygdalina for 48 hrs before transfer to V. amygdalina-free medium was not significantly different (P > 0.05) from untreated cells. These results suggest that V. amygdalina, at least at concentrations up to 10 mg/ml, exhibits cytostatic action to retard the growth of human breast cancer cells. In addition, the ERK signaling pathways may be one or more of the intracellular targets for V. amygdalina antineoplastic actions.
最近有报道称，可食用的扁桃斑鸠菊叶的水溶性提取物是培养的MCF - 7细胞的有效抑制剂。此前尚未研究过扁桃斑鸠菊抑制MCF - 7细胞生长的机制。本研究的目的是评估扁桃斑鸠菊对细胞外信号调节蛋白激酶1和2（ERK 1/2）的活性、DNA合成及后续细胞生长的影响。用不同浓度（3 - 100 mg/ml）的扁桃斑鸠菊水溶性提取物处理细胞，无论有无血清，均以浓度依赖的方式有效抑制ERK活性、DNA合成（P < 0.005）和细胞生长（P < 0.01）。在转移到不含扁桃斑鸠菊的培养基之前，用10 mg/ml扁桃斑鸠菊预处理细胞48小时，其生长速率与未处理细胞相比无显著差异（P > 0.05）。这些结果表明，扁桃斑鸠菊至少在浓度高达10 mg/ml时，表现出抑制细胞生长的作用，以延缓人乳腺癌细胞的生长。此外，ERK信号通路可能是扁桃斑鸠菊抗肿瘤作用的一个或多个细胞内靶点。
