Oikpefan Emmanuel Oise, Ayinde Buniyamin Adesina, Mudassir Azhar, Farooq Ahsana Dar
Delta State University, Faculty of Pharmacy, Department of Pharmacognosy and Traditional Medicine, Abraka, Nigeria.
University of Benin, Faculty of Pharmacy, Department of Pharmacognosy, Benin City, Nigeria.
Turk J Pharm Sci. 2019 Dec;16(4):375-379. doi: 10.4274/tjps.galenos.2018.19942. Epub 2019 Nov 11.
Mill. I.M.Johnst. is a medicinal plant widely used in ethnomedicine for the treatment of cancer and other diseases.
The effects of methanol extracts of the leaf, stem, and root barks were evaluated on breast (MCF-7) and lung (NCI-H460) cancer cells at 1-250 μg/mL using the SRB assay and the extracts were screened for phytochemicals using the standard method.
The stem and root extracts showed no activity at the maximum concentration, while the leaf extract at 100 μg/mL showed remarkable cell growth inhibition against breast (-14.50±0.58) and lung cancer (+53.29±4.57) in vitro. The extracts showed the presence of saponins, terpenes, cardiac glycosides, and phenolic compounds. Partitioning of the active leaf extract further enhanced its activity as the chloroform fraction exhibited GI, LC, and total growth inhibition (TGI) of 22.5, 68.75, and 43.75 μg/mL against breast cancer, respectively, and GI and TGI of 35.4 and 55.8 μg/ mL against lung cancer cells, respectively. However, the aqueous fraction showed no cytotoxicity against either cell line.
These results justified the ethnomedicinal uses of the plant against tumor-related ailments. Isolation of the constituents responsible for the observed activity needs to be carried out to further support this claim.
千屈菜是一种药用植物，在民族医学中广泛用于治疗癌症和其他疾病。
采用SRB法评估叶、茎和根皮的甲醇提取物在1 - 250μg/mL浓度下对乳腺癌（MCF - 7）和肺癌（NCI - H460）细胞的作用，并使用标准方法对提取物进行植物化学成分筛选。
茎和根提取物在最大浓度下无活性，而叶提取物在100μg/mL时对体外乳腺癌（-14.50±0.58）和肺癌（+53.29±4.57）显示出显著的细胞生长抑制作用。提取物中含有皂苷、萜类、强心苷和酚类化合物。活性叶提取物的分配进一步增强了其活性，氯仿部分对乳腺癌的GI、LC和总生长抑制（TGI）分别为22.5、68.75和43.75μg/mL，对肺癌细胞的GI和TGI分别为35.4和55.8μg/mL。然而，水相部分对两种细胞系均无细胞毒性。
这些结果证明了该植物在民族医学中用于治疗肿瘤相关疾病的合理性。需要对具有观察到活性的成分进行分离，以进一步支持这一说法。
