Choquet Gwénaëlle, Soudant Philippe, Lambert Christophe, Nicolas Jean-Louis, Paillard Christine
Laboratoire des Sciences de l'Environnement Marin (LEMAR), Institut Universitaire Européen de la Mer (IUEM-UBO), Place Copernic Technopôle Brest-Iroise, 29280 Plouzané, France.
Dis Aquat Organ. 2003 Dec 3;57(1-2):109-16. doi: 10.3354/dao057109.
Vibrio tapetis is the causative agent of brown ring disease (BRD), which affects a species of clam, Ruditapes philippinarum. After incubation with V. tapetis, hemocytes lose filopods and become rounded, indicating cytotoxic activity of the bacterium. To rapidly quantify this cytotoxicity, a flow-cytometry test was developed based on the capacity of V. tapetis to inhibit adhesion of clam hemocytes to plastic. Several bacteria:hemocyte ratios, the cytotoxicity of other Vibrio spp. pathogenic to bivalves, and that of various V. tapetis isolates were tested. Inhibition of adherence is detectable with as few as 5 bacteria per hemocyte. The greater cytotoxic activity of V. tapetis compared to that of V. splendidus and V. pectenicida suggests a specific pathogenicity of V. tapetis to R. philippinarum hemocytes. Although all V. tapetis isolates inhibited adhesion, significant variations in cytotoxicity among isolates was demonstrated.
塔氏弧菌是褐环病（BRD）的病原体，该病影响菲律宾蛤仔这一蛤类物种。与塔氏弧菌孵育后，血细胞失去丝状伪足并变得圆润，表明该细菌具有细胞毒性活性。为了快速量化这种细胞毒性，基于塔氏弧菌抑制蛤血细胞与塑料黏附的能力开发了一种流式细胞术检测方法。测试了几种细菌与血细胞的比例、其他对双壳贝类致病的弧菌属细菌的细胞毒性以及各种塔氏弧菌分离株的细胞毒性。每个血细胞中低至5个细菌就能检测到黏附抑制。与灿烂弧菌和栉孔扇贝弧菌相比，塔氏弧菌具有更强的细胞毒性活性，这表明塔氏弧菌对菲律宾蛤仔血细胞具有特异性致病性。尽管所有塔氏弧菌分离株均抑制黏附，但各分离株之间的细胞毒性存在显著差异。
