IUEM, UMR CNRS 6539, Technopôle Brest Iroise, 29280 Plouzané, France.
Fish Shellfish Immunol. 2011 Dec;31(6):1137-41. doi: 10.1016/j.fsi.2011.10.008. Epub 2011 Oct 14.
The Manila clam, Ruditapes philippinarum can become infected by the bacterium Vibrio tapetis which causing the Brown Ring Disease along North European Atlantic coasts. Variations in clam immune parameters have been reported in clam challenged with V. tapetis but no studies have been done on Nitric Oxide (NO) production. NO is a toxic agent to pathogens produced mostly by immune cells such as hemocytes in invertebrates. In this study, we demonstrated that NO production in hemolymph and extrapallial fluid of clams is dose dependent and increases with incubation time with V. tapetis. Moreover, the augmentation of NO production seems to be directly correlated to cell rounding and to the loss of pseudopods-forming capacity of hemocytes during the infection process.
菲律宾蛤仔（Ruditapes philippinarum）可感染导致北欧大西洋沿海地区出现棕环病的创伤弧菌（Vibrio tapetis）。在受到创伤弧菌刺激的蛤仔中，已经报道了蛤仔免疫参数的变化，但尚未对一氧化氮（NO）的产生进行研究。NO 是一种由免疫细胞（如无脊椎动物的血细胞）产生的对病原体有毒的物质。在这项研究中，我们证明了血液和外套膜外液中 NO 的产生与创伤弧菌的剂量有关，并随与创伤弧菌的孵育时间而增加。此外，NO 产生的增加似乎与细胞圆化以及血细胞在感染过程中丧失伪足形成能力直接相关。
