Hameed A S Sahul, Balasubramanian G, Musthaq S Syed, Yoganandhan K
Department of Zoology, C. Abdul Hakeem College, Melvisharam 632 509, Vellore District, Tamil Nadu, India.
Dis Aquat Organ. 2003 Dec 3;57(1-2):157-61. doi: 10.3354/dao057157.
Twenty species of Indian marine crabs were experimentally infected with white spot syndrome virus (WSSV), via the oral route and intramuscular injection, to determine their viral susceptibility. We determined that 16 species (Calappa philargius, Charybdis annulata, C. lucifera, Doclea hybrida, Grapsus albolineatus, Halimede ochtodes, Liagore rubronaculata, Lithodes maja, Matuta miersi, Paradorippe granulata, Parthenope prensor, Philyra syndactyla, Podophthalmus vigil, Portunus sanquinolentus, Scylla serrata and Thalamita danae) were susceptible and 4 (Atergatis integerrimus, Charybdis natator, Demania splendida or Menippe rumphii) were refractive at 50 d post-infection (p.i.). The presence of WSSV in these crabs was confirmed by PCR tests, histology and bioassay. WSSV was found in the gill, heart, eyestalks, striated muscle and cephalothoraxic tissue. The 4 WSSV-refractive species represent potential reservoirs or carriers of WSSV.
通过口服途径和肌肉注射，对20种印度海洋蟹进行了感染白斑综合征病毒（WSSV）的实验，以确定它们对该病毒的易感性。我们确定，16种蟹（红线黎明蟹、环纹蟳、锈斑蟳、杂色蛙蟹、白线紧握蟹、黄斑Halimede、红斑栗壳蟹、欧洲黄道蟹、密氏互敬蟹、颗粒毛刺蟹、强额互敬蟹、合指相手蟹、锐齿蟳、红肉河蟹、锯缘青蟹和达纳硬壳蟹）易感，4种蟹（正直爱洁蟹、武士蟳、光辉鲟或隆背哲蟹）在感染后50天表现为抗性。通过PCR检测、组织学和生物测定法证实了这些蟹中存在WSSV。在鳃、心脏、眼柄、横纹肌和头胸部组织中发现了WSSV。这4种对WSSV有抗性的蟹类代表了WSSV的潜在储存宿主或携带者。
